AGL Antibody(Center)
Cat.#: T0771
Rabbit Polyclonal Antibody
Clone ID: polyclonal
Swiss Prot: P35573
Mol Weight: 175kDa
Size: 100ug

Description
AGL is a glycogen debrancher enzyme which is involved in glycogen degradation. This enzyme has two independent catalytic activities which occur at different sites on the protein: a 4-alpha-glucotransferase activity and a amylo-1,6-glucosidase activity. Mutations in the AGL gene are associated with glycogen storage disease although a wide range of enzymatic and clinical variability occurs which may be due to tissue-specific alternative splicing.

Recommended Applications
WB, ELISA

Applications and Recommended Dilution Factors
WB: 1:50~100
ELISA: 1:1,000

Species Reactivity
Human

Products Data

 
Western blot analysis of hAGL-C371 in K562 cell line lysates (35ug/lane). AGL (arrow) was detected using the purified Pab.

Specificity
This AGL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 364~394 amino acids from the center region of human AGL.

Storage Condition and Buffer
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Alternative Names
AGL antibody, GDE antibody, Amylo-alpha-1,6-glucosidase antibody, Dextrin 6-alpha-D-glucosidase antibody

Description References
Horinishi, A., et al., J. Hum. Genet. 47(2):55-59 (2002).
Bao, Y., et al., Genomics 38(2):155-165 (1996).
Yang, B.Z., et al., J. Biol. Chem. 267(13):9294-9299 (1992).
Yang-Feng, T.L., et al., Genomics 13(4):931-934 (1992).
Bao, Y., et al., Gene 197 (1-2), 389-398 (1997). 

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