MouseAntiHumanInterleukin-10（IL-10）,蛋白质/抗原/多肽

IL10 is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.

Clone

NYR-HIL10.

Immunogen

r.Human IL-10.

Ig Subclass

Mouse IgG1.

Purification Method

Ion excahnge.

Protein concentration

1mg/ml in PBS (after reconstitution).

Shipping Conditions

Antibody is shipped lyophilized at ambient temperature.

Storage Procedures

In lyophilized form, for long periods, store at 4oC in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20oC.

Reconstitution

Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Stability / Shelf LifeTwo years lyophilized, one month in solution at 4oC.

Applications

Direct ELISA, Western Blot, Immuneprecipitation, Immunohistochemistry, Intracellular staining.

Titer

By direct ELISA, 1:20,000 dilution will yield >1.0 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories).

Usage

Prospec's products are furnished for LABORATORY ESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.