其它生化试剂
MouseAntiFLAGpeptide
| 品牌 | 厂商性质 | 产地 | 货期 |
|---|---|---|---|
| Prospec | 暂无 | 暂无 | 现货 |
产品介绍
| Catalogue Number | ANT-222 | ||
| Type | Mouse Antibody Monoclonal. | ||
| Introduction | A fusion tag called FLAG consists of eight amino acids (AspTyrLysAspAspAspAspLys) including an enterokinase-cleavage site. FLAG is specifically designed for immunoaffinity chromatography. It allows elution under non-denaturing conditions. Several antibodies against this peptide have been developed. One antibody denoted as M1 binds the peptide in the presence of bivalent metal cations preferably Ca(+). Elution is effected by chelating agents. Another strategy is competitive elution with excess of free Flag Peptide. The FLAG peptide purifies and detects recombinant fusion proteins. FLAG peptide is useful in Western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization. Flag peptide is a hydrophilic tag which significantly improves the detection and purification of recombinant fusion proteins. | ||
| Clone | PFLAGSHG. | ||
| Description | Monoclonal antibodies are produced by immunizing mouse with synthetic peptide (DYKDDDDK), coupled to KLH. | ||
| Ig Subclass | Mouse IgG2b. | ||
| Formulation | 1×PBS & 50% glycerol. | ||
| Stability / Shelf Life | 2 years at -20°C. 1 month at 4°C. | ||
| Dilution | Western Blotting: 0.5µg/ml. | ||
| Applications | Western Blot, Immunoprecipitation. | ||
| Usage | ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. | ||
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注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途