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酶·辅酶

Hydroxysteroid Dehydrogenase from Pseudomonas testosteroni

品牌 厂商性质 产地 货期
阿拉丁 生产商 上海 现货

询价
产品参数
货号 操作
H128453-1g 询价
H128453-5g 询价
H128454-10UN 询价
H128454-50UN 询价
产品介绍

  • 分子式
  • 分子量
  • Beilstein号
  • EC号
  • MDL号
  • PubChem编号

属性

存贮条件 储存温度 -20°C

描述

产品介绍 Hydroxysteroid dehydrogenases catalyze the interconversion of hydroxyl and carboxyl groups of steroids. P.testosteroni derived hydroxysteroid dehydrogenases are of two types: 3-α-hydroxysteroid dehydrogenase (α enzyme) and 3-β-hydroxysteroid dehydrogenase (β enzyme). The α enzyme has a molecular weight of 47,000 daltons. The α enzyme oxidizes only 3-α-hydroxysteroids of the C19, C21 and C24 series. It is inhibited by heavy metals and sulfhydryl-binding agents. The β enzyme catalyzes the oxidation of 3-β-hydroxysteroids of the C19 and C21 series, 17-β-hydroxysteroids of the C18, C19 and C21 series, as well as certain 16-β-hydroxysteroids. It is inhibited by heavy metals and reducing agents. The oxidation of testosterone is inhibited by 3,17-α-estradiol and other 1,3,5-estradiene derivatives. Aladdin supplies two preparations: one from the regular P. testosteroni (ATCC 11966) culture which produces both the α and the β enzymes, and a second from a mutant strain which produces almost exclusively the α enzyme. By using both, the β-hydroxysteroid can be determined by difference.
备注 A purified powder obtained from induced cells. Contains both alpha and beta activities.
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