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酶·辅酶
己糖激酶 来源于酵母(冻干)
| 品牌 | 厂商性质 | 产地 | 货期 |
|---|---|---|---|
| 阿拉丁 | 生产商 | 上海 | 现货 |
产品参数
| 货号 | CAS号 | 操作 |
|---|---|---|
| H128554-10KU | 9001-51-8 | 询价 |
| H128554-2.5KU | 9001-51-8 | 询价 |
产品介绍
- 分子式
- 分子量
- Beilstein号
- EC号 232-611-5
- MDL号 MFCD00131321
- PubChem编号
属性
| 敏感性 | 易吸潮 |
| 存贮条件 | 储存温度 -20°C |
描述
| 应用 | Catalyzes the phosphorylation of D-hexose sugars at the C6 position utilizing ATP as a phosphate source. Used to determine glucose, fructose, mannose and ATP. |
| 产品介绍 | Hexokinase catalyzes the reaction:D-hexose + ATP → D-hexose 6-phosphate + ADP Hexokinases have been isolated from the yeast cell in two distinct forms, designated P-I and P-II (Schulze et al. 1969). These are separate, noninterconvertible isozymes (Womack et al. 1973). Hexokinase is used to determine glucose, fructose, mannose and ATP. |
| 备注 | Chromatographically purified. A lyophilized powder containing ~30% buffer/stabilizer. Phosphohexose isomerase, 6-phosphogluconate dehydrogenase, adenylate kinase and creatine phosphokinase contaminant activities are 0.10%, 0.005%, 0.005% and 0.005%, respectively. |
| 生化机理 |
Catalyzes the phosphorylation of D-hexose sugars at the C6 position utilizing ATP as a phosphate source. The rate of phosphorylation varies with different hexoses (pH 7.5, 30 °C):D-fructose KM: 0.33 mM;D-glucose KM: 0.12 mM;D-mannose KM: 0.05 mM. Yeast hexokinase exists as two similar isoforms, PI and PII (A and B), with isoelectric points of 5.25 and 4, respectively.Molecular Weight: ~ 54 kDa (monomer);~110 kDa (dimer);Optimal pH: 7.5 to 9.0;Extinction Coefficient: E1% = 8.85 (PI) and 9.47 (PII) at 280 nm. Activators: Hexokinase requires Mg2+ ions (KM = 2.6 mM) for activity. Hexokinase is activated by catecholamines and related compounds. Inhibitors: sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxy-methylglucose?, xylose, lyxose, and thiol reactive compounds (Hg2+ and 4-chloromercuribenzoate). |
| 别名 | 己糖激酶;ATP:D-Hexose-6-phosphotransferase;Hexokinase from Saccharomyces cerevisiae;Hexokinase |
己糖激酶 来源于酵母(冻干)信息由上海阿拉丁生化科技股份有限公司为您提供，如您想了解更多关于己糖激酶 来源于酵母(冻干)报价、型号、参数等信息，欢迎来电或留言咨询。
注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途