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酶·辅酶
胰蛋白酶 来源于牛胰脏
| 品牌 | 厂商性质 | 产地 | 货期 |
|---|---|---|---|
| 阿拉丁 | 生产商 | 上海 | 现货 |
产品参数
| 货号 | CAS号 | 操作 |
|---|---|---|
| T105533-100g | 9002-07-7 | 询价 |
| T105533-25g | 9002-07-7 | 询价 |
| T105533-5g | 9002-07-7 | 询价 |
产品介绍
- 分子式
- 分子量
- Beilstein号
- EC号 232-650-8
- MDL号 MFCD00082094
- PubChem编号
属性
| 敏感性 | 对光和湿度敏感 |
| 溶解性 | Soluble in Hank's Balanced Salt Solution (35 mg/ml), HCl (1 mM), and water. Insoluble in clycerol, and alcohol. |
| 存贮条件 | 储存温度-20°C |
描述
| 应用 | or trypsin digestion of peptides, use a ratio of about 1:100 to 1:20 for trypsin:peptide. The typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Trypsins have also been used for the re-suspension of cells during cell culture, in proteomics research for digestion of proteins and in various in-gel digestionsns?. Additional applications include assessing crystallization by membrane-based techniques and in a study to determine that protein folding rates and yields can be limited by the presence of kinetic traps. |
| 产品介绍 | 胰蛋白酶是一种丝氨酸蛋白水解酶,属白色或微黄色冻干粉制品。它能特异性地水解碱性氨基酸精氨酸及赖氨酸羧基所组成的肽键。酶本身很容易自溶，其作用最佳的PH值为8.0～9.0。胰蛋白酶活性能被胰腺中的天然抑制剂所抑制。钙离子能延迟胰蛋白酶的自溶，并能促进胰蛋白酶原的活性。 |
| 生化机理 | Trypsin cleaves peptides on the C-terminal side of lysine and arginine residues. The rate of hydrolysis of this reaction is slowed if an acidic residue is on either side of the cleavage site and hydrolysis is stopped if a proline residue is on the carboxyl side of the cleavage site. The optimal pH for trypsin activity is 7-9. Trypsin can also act to cleave ester and amide linkages of synthetic derivatives of amino acids. EDTA is added to trypsin solutions as a chelating agent that neutralizes calcium and magnesium ions that obscure the peptide bonds on which trypsin acts. Removing these ions increases the enzymatic activity. |
| 别名 | ; |
胰蛋白酶 来源于牛胰脏信息由上海阿拉丁生化科技股份有限公司为您提供，如您想了解更多关于胰蛋白酶 来源于牛胰脏报价、型号、参数等信息，欢迎来电或留言咨询。
注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途