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酶·辅酶
蛋白内切酶 Glu-C 来源于金黄色葡萄球菌 V8
| 品牌 | 厂商性质 | 产地 | 货期 |
|---|---|---|---|
| 阿拉丁 | 生产商 | 上海 | 现货 |
产品参数
| 货号 | CAS号 | 操作 |
|---|---|---|
| P128655-5mg | 66676-43-5 | 询价 |
| P128655-1mg | 66676-43-5 | 询价 |
产品介绍
- 分子式
- 分子量
- Beilstein号
- EC号
- MDL号 MFCD01324998
- PubChem编号
属性
| 存贮条件 | 储存温度 2-8°C |
描述
| 应用 | Endoproteinase Glu-C from Staphylococcus aureus strain V8 is a serine protease used for selective cleavage of proteins for amino acid sequence determination or peptide mapping . |
| 产品介绍 | Protease S. aureus V8 (Endoproteinase-Glu-C) specifically cleaves peptide bonds on the COOH-terminal side of either aspartic or glutamic acids. In the presence of ammonium, the enzyme specificity is limited to glutamic sites. It has a molecular weight of 27,000 daltons and optimum pH's of 4.0 and 7.8 with hemoglobin as the substrate. Protease S. aureus V8 is inhibited by diisopropylfluorophosphate and monovalent anions such as F-, Cl-, CH3COO- and NO3. Enzyme activity is determined by the casein digestion assay described by Drapeau (Methods Enzymol., 45, 469, 1976). |
| 备注 | Chromatographically purified according to Drapeau, G., Boily, Y., and Houmard, J.: JBC, 247, 6720 (1972). A lyophilized powder. |
| 生化机理 | Staphylococcus strain V8 protease specifically cleaves peptide bonds on the carboxyl side of aspartic and glutamic acid residues when used in phosphate buffer. When used in ammonium bicarbonate buffer or ammonium acetate buffer cleavage is restricted to the carboxyl side of glutamic acid residues only. The enzyme exhibits maximal activity from pH 4.0 to 7.8. If hemoglobin is used as the substrate, maximal activity is at pH 4.0. The maximal activity is at pH of 7.8 when casein is the substrate. |
| 别名 | V8蛋白酶;V8 Protease;Protease,Staph aureus from Staphylococcus aureus V8 |
蛋白内切酶 Glu-C 来源于金黄色葡萄球菌 V8信息由上海阿拉丁生化科技股份有限公司为您提供，如您想了解更多关于蛋白内切酶 Glu-C 来源于金黄色葡萄球菌 V8报价、型号、参数等信息，欢迎来电或留言咨询。
注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途