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其它细胞培养
外源凝集素(菜豆)
| 品牌 | 厂商性质 | 产地 | 货期 |
|---|---|---|---|
| 阿拉丁 | 生产商 | 上海 | 现货 |
产品参数
| 货号 | 操作 |
|---|---|
| P140098-5mg | 询价 |
产品介绍
属性
| 敏感性 | 对湿度敏感 |
| 存贮条件 | 储存温度-20°C |
描述
| 应用 | Erythrocyte agglutination studies;Model system of how proteins recognize carbohydrates |
| 产品介绍 | Phaseolus vulgaris lectin (PHA-E) is isolated from red kidney beans and purified by affinity chromatography and ion-exchange chromatography. It is a tetrameric protein with a molecular weight of 128 kDa. The lectin recognizes and binds specifically to terminal galactose, N-acetylglucosamine and mannose residues of complex glycans on mammalian glycoproteins. Because of its strong erythrocyte agglutinating activity, it is also known as Erythroagglutinin (PHA-E). PHA-E is not blood group specific. Less than 5 μg/ml agglutinates human erythrocytes of type O and agglutination can be inhibited by certain oligosaccharides. PHA-E lectin is supplied as a white lyophilized powder from a buffer containing from 50 mM NH4HCO3. The purity of the lectin in determined by SDS-PAGE, which generate one single band at 34 kDa. |
| 备注 | The lectin may be reconstituted with 2 ml of deionized water before use. Spin the vial gently until full dissolution. Aggregation is thought to occur in the presence of high concentrations of 2-mercaptoethanol. The solution may be reconstituted in this buffer to desired working concentration. |
| 生化机理 |
PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity; PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. |
| 别名 | 植物血球凝集素；红腰豆/菜豆凝集素;Lectin from Phaseolus vulgaris (red kidney bean);Phaseolus vulgaris agglutinin; PHA |
外源凝集素(菜豆)信息由上海阿拉丁生化科技股份有限公司为您提供，如您想了解更多关于外源凝集素(菜豆)报价、型号、参数等信息，欢迎来电或留言咨询。
注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途