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3-氨基-9-乙基咔唑

品牌 厂商性质 产地 货期
阿拉丁 生产商 上海 现货

询价
产品参数
货号 CAS号 价格(元) 操作
A105545-25g 132-32-1 499 询价
A105545-5g 132-32-1 149 询价
产品介绍

  • 分子式 C14H14N2
  • 分子量210.28
  • Beilstein号 22(4)4936
  • EC号
  • MDL号 MFCD00004964
  • PubChem编号

属性

熔点 98°C-100°C
存贮条件 储存温度2-8℃
密度 1.1700

描述

应用 过氧化物酶底物.
Usage
Prepare samples and incubate with antibodies and HRP-labeled probes, follow with all necessary wash steps.
? Prepare 1X AEC Substrate Buffer (dilute AEC Substrate Buffer, 10X;).
? Dilute one part AEC, 50X with 50 parts 1X AEC Substrate Buffer to make 1X AEC Substrate Solution. Mix well.
? Cover tissue sections with 1X AEC Substrate Solution and incubate 10-30 min. at RT.
? Rinse thoroughly with distilled water. If desired, follow with an appropriate aqueous-based counterstain.
? Mount tissue samples with an aqueous-based mounting medium (e.g., Crystal Mounting Medium; )
? Unused AEC solution can be discarded according to city, county, state, province or country’s regulations
产品介绍 AEC, 50X (3-amino-9-ethylcarbazole); for HRP-based detection systems; provides red chromogen depositions. Precipitable peroxidase substrate for immunohistochemical (IHC) staining techniques. In the presence of horseradish peroxidase (HRP), produces a water-insoluble red oxidation product. Although not as sensitive as diaminobenzidine (DAB, 50X;, the red deposit formed at the sites of AEC oxidation make this substrate useful for double labeling. Aqueous-based mounting medium and counterstains must be used for IHC applications since oxidized AEC is soluble in alcohols and xylene (e.g., Crystal Mounting Medium; ). Use with AEC Substrate Buffer .
生化机理 3-氨基-9-乙基咔唑是一种过氧化酶底物,适用于免疫印迹和免疫组织化学染色程序。该底物能产生一种不溶性红色的末端产物。肉眼或可见光下就可观察。
别名 AEC, 50X ;3-氨基-N-乙基咔唑;AEC, 50X

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注:该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案,不可用于临床诊断或治疗等相关用途