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杂环砌块
碘硝基四唑紫（INT）
| 品牌 | 厂商性质 | 产地 | 货期 |
|---|---|---|---|
| 阿拉丁 | 生产商 | 上海 | 现货 |
产品参数
| 货号 | CAS号 | 价格（元） | 操作 |
|---|---|---|---|
| I105499-1g | 146-68-9 | 379 | 询价 |
| I105499-250mg | 146-68-9 | 128 | 询价 |
| I105499-25g | 146-68-9 | 6399 | 询价 |
| I105499-5g | 146-68-9 | 1549 | 询价 |
产品介绍
- 分子式 C19H13N5O2ICl
- 分子量505.71
- Beilstein号 4.09322e+006
- EC号 MFCD00011961
- MDL号 MFCD00011961
- PubChem编号
属性
| 熔点 | 231°C |
| 敏感性 | 易吸潮,对热及光线敏感 |
| 溶解性 | Soluble in water (4 mg/ml), ethanol (8 mg/ml), ethylene glycol (30 mg/ml), DMSO (slightly), and methanol: water (1:1, 50 mg/ml, very faintly turbid, very deep yellow,hot). |
| 存贮条件 | 储存温度2-8℃ |
描述
| 应用 | 色度法检测各种脱氢酶的电子受体（Electron acceptor）碘硝基四唑紫已用于细胞染色。它已被用作 D-阿拉伯糖醇的酶促测定中的偶联剂。 |
| 产品介绍 | Iodonitrotetrazolium chloride is an electron acceptor used in various colorimetric assays. The chemical has been mainly documented for its measurement of dehydrogenase activity. In the assay to measure SDH (succinate dehydrogenase) activity, Iodonitrotetrazolium chloride was used as an oxidizing agent being reduced by succinate dehydrogenase. Upon reduction the compound forms a formazan dye measured at 500 nm. Iodonitrotetrazolium chloride has also been used to study the electron transport activity in soils, sediments, and pure enzyme cultures as well as nucleotide diphosphate kinase activity. |
| 生化机理 | spectrophotometric assay for succinate dehydrogenase activity: Materials: spectrophotometer cuvette with 1-cm light patch. Reaction Mixture: 100 mmol/liter triethanolamine (HCl), pH8,3 o.5 mmol/liter EDTA, 2 mmol/liter Iodonitrotetrazolium chloride, 12 g/liter Cremophor EL, 20 mmol/liter succinate, pH7.4 Reaction: mix 990 ul of reaction mixture with 10ul mitochondrial suspension color develops at 30oC record for 6 min. @500 nm |
| 别名 | 碘硝基氯化四氮唑蓝;2-(对碘苯基)-3-(对硝基苯基)-5-苯基四唑氯水合物;2-(4-碘苯基)-3-(4-硝基苯基)-5-苯基氯化四唑氯水合物;碘硝基氯化四氮唑;INT;对碘硝基四唑紫;2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride; p-Iodonitrotetrazolium Violet; INT Iodonitrotetrazolium chloride |
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注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途