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Immunogen

N-terminal synthetic decapeptide of α-smooth muscle actin.

Other Notes

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide, as a preservative.

Application

Immunocytochemistry was performed on smooth muscle cells from bovine aortas using the monoclonal anti-ACTA2 antibody. Cells were first grown on glass cover slips and fixed in 50% acetone/EtOH for 10 minutes at 4 degrees.

Monoclonal Anti-Actin, α-Smooth Muscle - FITC antibody is suitable for:
• immunohistochemical (IHC) staining of heart, lung, kidney, and liver of mice to examine the PHD2 deficiency in internal organs^[2]
• immunostaining to analyze vascular smooth muscle cells/pericytes development in platelet-derived growth factor (PDGFR) and PDGFR- β-/- embryos^[3]
• immunofluorescent analysis of implanted cells, frozen tissue samples to study the similar levels of expression of SMaA and SM-MHC as established primary SMC lines^[4]
• western blots of lens epithelial cells to determine the distribution of α-SMA expression in lens epithelial cell^[7]

Paraffin embedded sections of rat testis tissue grafts were immunohistochemically stained with mouse monoclonal anti-smooth muscle actin. 
IHC analysis of x-gal stained muouse cardiac tissue was performed using the primary antibody, mouse monoclonal anti-smooth muscle actin to identify myofibroblasts.

Biochem/physiol Actions

Actin, a conserved eukaryotic protein, is the major cytoskeletal component which actively participates in cell motility. In mammals and birds, actin is expressed as six different isoforms.^[5][6] α-Smooth muscle actin, a smooth muscle cell-specific protein, is also known as aortic smooth muscle or α smooth muscle actin (α-SMA, SMactin, α-SM-actin, ASMA). It belongs to actin family, which are highly conserved proteins that play a role in cell motility, structure and integrity. It is predominantly found in cells of the smooth muscle lineages. It has been reported that α-smooth muscle actin was also found in certain non-muscle cells, in particular fibroblasts i.e. myofibroblasts.^[1]

General description

The antibody (also known as anti-α-Sm-1) is specific for the single isoform of α-smooth muscle actin. It reacts specifically with α-smooth muscle actin in immunoblotting assays and labels smooth muscle cells in frozen or formalin-fixed, paraffin-embedded tissue sections.

性质

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