聚乙二醇

Poly(ethylene glycol)

BioUltra, for molecular biology, 1,000

产品描述

Other Notes

PEG precipitation of DNA fragments^[5]

Application

Poly(ethylene glycol) has been used to develop a method for efficient transformation of Brevibacterium lactofermentum protoplasts with plasmid DNA.^[1]It has been used as a fusion agent to fuse spleen cells with the myeloma cell line SP2/0.^[2]

General description

Poly(ethylene glycol) (PEG) is a non-ionic hydrophilic polymer and is available in different molecular weights. It helps in the purification and crystal growth of proteins and nucleic acids. PEG and dextran together result in aqueous polymer two phase system, which is required for the purification of biological materials. PEG also interacts with cell membrane, thereby allowing cell fusion.^[3][4]

 性质

Related CategoriesBioUltra, Essential Chemicals, Optimization Reagents, PEG and Oligo Ethylene Glycol,Poly(ethylene glycol),

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grade  for molecular biology

vapor density  >1 (vs air)

vapor pressure  <0.01 mmHg ( 20 °C)

product line  BioUltra

form  solid

autoignition temp.  581 °F

mol wt  M_r 950-1050

impurities  DNases, none detected

 RNases, none detected

 insoluble matter, passes filter test

 phosphatases, none detected

 proteases, none detected

pH  5.5-7.0 (25 °C, 50 mg/mL in H₂O)

solubility  H₂O: 50 mg/mL at 20 °C, clear, colorless

anion traces  chloride (Cl^-): ≤50 mg/kg

 sulfate (SO₄^2-): ≤50 mg/kg

cation traces  Al: ≤5 mg/kg

 As: ≤0.1 mg/kg

 Ba: ≤5 mg/kg

 Bi: ≤5 mg/kg

 Ca: ≤10 mg/kg

 Cd: ≤5 mg/kg

 Co: ≤5 mg/kg

 Cr: ≤5 mg/kg

 Cu: ≤5 mg/kg

 Fe: ≤5 mg/kg

 K: ≤200 mg/kg

 Li: ≤5 mg/kg

 Mg: ≤5 mg/kg

 Mn: ≤5 mg/kg

 Mo: ≤5 mg/kg

 Na: ≤200 mg/kg

 Ni: ≤5 mg/kg

 Pb: ≤5 mg/kg

 Sr: ≤5 mg/kg

 Zn: ≤5 mg/kg

λ  50 mg/mL in H₂O

UV absorption  λ: 260 nm A_max: 0.06

 λ: 280 nm A_max: 0.03

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