A3437 SIGMA

Anti-Human IgM (μ-chain specific)−Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous glycerol solution

• MDL number MFCD00162460

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  Properties

  Related Categories	Alkaline Phosphatase, Alphabetical Index,Antibodies, HU-HZ, Human IgM Secondary Antibodies and Conjugates, More...
  biological source	goat
  antibody form	affinity isolated antibody
  clone	polyclonal
  form	buffered aqueous glycerol solution
  species reactivity	human
  application(s)	direct ELISA: 1:30,000
  	dot blot: 1:30,000

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  Description

  Immunogen

  Human IgM

  Physical form

  Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl₂, 10 mM glycine, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide

  Application

  Anti-Human IgM (μ-chain specific)-Alkaline Phosphatase antibody is suitable for use in ELISA^[5][1].

  Goat Anti-Human IgM (μ-chain specific)-Alkaline Phosphatase antibody can be used for dot blot and western blot applications at 1:30,000.

  Goat polyclonal anti-Human IgM (μ-chain specific)−Alkaline Phosphatase antibody may be used to detect and quantitate the level of IgM in human serum and biological fluids via chromogenic, chemoluminescent or fluorogenic immunochemical or immunohistochemical techniques.

  IgM is a glycoprotein with 5 n-linked glycosylation sites on the heavy chain. An ELISA assay was performed to identify glycosylated forms of IgM that bind to lectin. Alkaline phosphatase conjugated goat anti-human IgM was used as the secondary at 1:2000 and developed using p-nitrophenyl substrate (Sigma).^[7]

  General description

  Human IgMs are the initial immunoglobulin isotypes that appear in blood in response to the first exposure to external antigens. These immunoglobulins have been implicated in CNS myelin repair. Increased levels of secretory IgM have been linked to rhino-conjunctivitis ^[4][2][3]. Anti-Human IgM (μ-chain specific)-Alkaline Phosphatase antibody is specific for human IgM when tested against human IgA, IgG, IgM, Bence Jones κ, and λ myeloma proteins.

  Immunoglobulin M (IgM) antibodies appear early in the course of infections. IgM antibodies are responsible for agglutination of red blood cells in mis-matched blood transfusions. The level of IgM may vary with the status of disease or infection. 
  Alkaline Phosphatase is an enzyme that catalyzes the conversion of chromogenic substrates such as p-nitrophenylphosphate (PNPP); chemiluminescent substrates such as CDP-Star^® and fluorogenic substrates such as 4-methylumbelliferyl phosphate (4-MUP) into detectable chromophores, light-emitters or fluorescers, respectively.

  Legal Information

  CDP-Star is a registered trademark of Tropix, Inc.

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