ANTI-FLAG? antibody produced in rabbit
| 品牌 | 厂商性质 | 产地 | 货期 |
|---|---|---|---|
| Sigma-Aldrich | 一般经销商 | 美国 | 现货 |
| 货号 | 规格 | 包装 | 操作 |
|---|---|---|---|
| F7425-.2MG | 瓶 | 0.20mg | 询价 |
产品描述
Application
The antibody recognizes the FLAG epitope located on FLAG-tagged fusion proteins at the N-terminus or C-terminus, applying dot blot, immunoblotting, immunoprecipitation and immunocytochemistry assays.
Browse additional application references in our FLAG® Literature portal.
Features and Benefits
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General description
The rabbit Anti-FLAG polyclonal affinity antibody ANTI-FLAG recognizes the FLAG epitope located on FLAG fusion proteins. This antibody reacts with N-terminal, N-terminal-Met, and C-terminal FLAG fusion proteins.
Immunogen
FLAG; peptide sequence DYKDDDDK
Preparation Note
Purified by affinity chromatography on a column bearing the immunizing peptide.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide
法律信息
FLAG is a registered trademark of Sigma-Aldrich Co. LLC
性质
| Related Categories | Antibodies and Conjugates, Antibodies to Peptide and Protein Fusion Tags, FK-FO, FLAG, FLAG Antibodies, |
| biological source | rabbit |
| antibody form | affinity isolated antibody |
| form | buffered aqueous solution |
| shelf life | 1 mo. at 2 - 8 °C |
| concentration | ~.8 mg/mL |
| application(s) | dot blot: 1-2.5 μg/mL |
| immunoprecipitation (IP): 4-8 μg using amino terminal FLAG-BAP fusion protein from E. coli crude lysate | |
| indirect immunofluorescence: 5-10 μg/mL using 293T cells transfected with a plasmid encoding FLAG-JNK | |
| western blot (chemiluminescent): 1-2.5 μg/mL using an E. coli periplasmic extract expressing an N-terminal FLAG fusion protein | |
| immunogen sequence | DYKDDDDK |
| conjugate | unconjugated |
| shipped in | dry ice |
| storage temp. | −20°C |
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【Sigma-Aldrich 中国代理商：联硕生物科技有限公司】
Sigma-Aldrich is a leading Life Science and High Technology company. Our chemical and biochemical products and kits are used in scientific research, including genomic and proteomic research, biotechnology, pharmaceutical development, the diagnosis of disease and as key components in pharmaceutical, diagnostic and other high technology manufacturing.
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注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途