Biochem/physiol Actions

Mode of Action: Binds to 70S ribosomal subunit; inhibits translocation; elicits miscoding. Antimicrobial spectrum: Gram-negative and Gram-positive bacteria, and mycoplasma.

Caution

Solutions are stable at 37°C for approximately 5 days. Aqueous stock solutions can be stored at 2-8°C for long term storage.

包装

1, 5, 25 g in poly bottle

Preparation Note

Kanamycin sulfate is soluble in water at 50 mg/mL, yielding a clear solution. It is practically insoluble in alcohol, acetone, chloroform, ether and ethyl acetate. A 1% solution in water has a pH of 6.5 to 8.5. Sterile solutions can be prepared by a sterile filtration, through a .2μm filter.

Application

Kanamycin sulfate from Streptomyces kanamyceticus has been used in tissue culture media.^[1]

General description

Chemical structure: aminoglycoside

在生物技术中用于抑制蛋白质合成。建议以 100mg/L 的量用于细胞培养应用。