Quantikine QuicKit 比色夹心法ELISA试剂盒
| 品牌 | 厂商性质 | 产地 | 货期 |
|---|---|---|---|
| R&D Systems | 生产商 | 美国 | 订货 |
性能特点
90分钟内获得结果的Quantikine ELISA
Get Quantikine quality in a fraction of the time!
Accomplish more in your day without compromising quality. The Quantikine QuicKit ELISAs provide quick, accurate quantitation of proteins in serum, plasma, and cell culture supernates. Unlike traditional ELISAs, QuicKit ELISAs have a fast, simplified protocol that only takes 90 minutes to results with just one wash step.
在很短的时间内获得Quantikine质量！
在不影响质量的情况下，在一天中完成更多工作。Quantikine QuicKit ELISA可快速准确地定量血清、血浆和细胞培养物上等物中的蛋白质。与传统的 ELISA 不同，QuicKit ELISA 具有快速、简化的协议，只需一个洗涤步骤即可获得 90 分钟的结果。
Features
Higher throughput — Results in 90 minutes
Simplified protocol — Only 1 wash step
Accurate quantitation
Long-term consistency and reproducibility
Guaranteed supply
Highly sensitive
特征
更高的吞吐量 — 在 90 分钟内获得结果
简化的实验方案 — 只需 1 个洗涤步骤
精确定量
长期一致性和可重复性
保证供应
高灵敏度
QuicKit ELISA Validated Sample Types
Quantikine QuicKit ELISAs are extensively optimized to reduce matrix effects that are commonly seen with complex sample matrices. The following sample types have been validated for use in these ELISA kits:
Serum
Plasma
Cell Culture Supernates
QuicKit ELISA 验证的样品类型
Quantikine QuicKit ELISA经过广泛优化，可减少复杂样品基质中常见的基质效应。以下样品类型已经过验证，可用于这些 ELISA 试剂盒：
血清
血浆
细胞培养上位物
QuicKit ELISA Kit Components
These colorimetric ready-to-use ELISAs come with all components necessary to run your assay. Kit contents may vary slightly. Please refer to product-specific inserts for a complete list of contents for each individual assay:
Anti-Tag coated plate
Tag-conjugated capture antibody concentrate
HRP-conjugated detection antibody concentrate
Assay Diluent
Calibrator Diluent
25X Wash Buffer
Substrate
Stop Solution
QuicKit ELISA 套件组件
这些比色即用型ELISA附带了运行测定所需的所有组件。套件内容可能略有不同。请参阅产品特定的插入物，了解每种测定的完整内容列表：
酶联免疫吸附测定原理
ELISA Assay Principle
这些测定利用抗标记涂层微孔板。将标准品、样品和质品加入平板中，然后加入抗体混合物。孵育1小时后，洗涤板，加入底物并孵育20分钟。然后加入停止溶液，并使用标准酶标仪读取板。
These assays utilize an anti-tag coated microplate. Standards, samples, and controls are added to plate, followed by subsequent addition of an antibody cocktail. Following a 1 hour incubation, plates are washed and substrate is added and incubated for 20 minutes. Stop solution is then added and plates are read using a standard microplate reader.
Data You can Trust
Although Quantikine® QuicKitTM ELISAs produce results in a fraction of the time of traditional ELISAs, quality data is not sacrificed for speed. QuicKits maintain similar standards for quality that you have come to expect from the gold-standard Quantikine ELISA brand.
您可以信赖的数据
虽然Quantikine QuicKit ELISA产生的结果时间只是传统ELISA的一小部分，质量数据不会为了速度而牺牲。QuicKits保持了您对黄金标准Quantikine ELISA品牌所期望的类似质量标准。
Linearity of Dilution
Linearity of dilution is a hallmark of the R&D Systems® Quantikine® ELISAs and QuicKits are no exception. Linearity is important for assessing matrix effects, confirming that the QuicKitTM ELISA accurately measures sample values. Poor linearity of dilution or extreme deviation from 100% indicates that matrix effects can artifactually enhance or interfere with analyte detection.
稀释的线性
稀释的线性度是研发系统Quantikine ELISA的标志，QuicKits也不例外。线性度对于评估矩阵效应非常重要，可以确认QuicKit ELISA可准确测量样品值。稀释度线性差或与 100% 的极端偏差表明基质效应会人为地增强或干扰分析物检测。
Figure 1. The IL-18 Quantikine® QuicKitTM ELISA has superior linearity to Brand A. At dilute concentrations, Brand A is susceptible to matrix effects.
图 1.The IL-18 Quantikine QuicKit ELISA具有优于品牌A的线性度。在稀释浓度下，品牌A容易受到基质效应的影响。
Figure 2. The R&D Systems® IP-10 Quantikine® QuicKitTM has superior linearity to the Brand A counterpart.
图 2. R&D Systems的IP-10 Quantikine QuicKit 具有优于品牌A对应物的线性度。
Figure 3. The R&D Systems® G-CSF Quantikine® QuicKitTM has superior linearity to the Brand A counterpart.
图 3. R&D Systems的 G-CSF Quantikine QuicKit 具有优于品牌A对应物的线性度。
Spike and Recovery 提取和回收
在提取和回收实验中，用感兴趣的重组靶分析物对天然样品进行加刺，以鉴定由样品基质引起的干扰。当样品中的物质干扰目标分析物的准确检测时，就会发生基质效应。基质效应是由非特异性抗体结合、抗体交叉反应性或干扰等生物现象引起的。峰值和恢复实验是评估测量准确性的重要工具。
In spike and recovery experiments, natural samples are spiked with the recombinant target analyte of interest to identify interference caused by sample matrices. Matrix effects occur when a substance in the sample interferes with the accurate detection of a target analyte. Matrix effects are caused by biological phenomena such as non-specific antibody binding, antibody cross reactivity or interference. Spike and recovery experiments are an important tool for assessing the accuracy of your measurement.
Figure 4. R&D Systems IL-18 Quantikine® QuicKitTM ELISAs have superior recovery to Brand A in serum and plasma.
图 4.R&D Systems的 IL-18 Quantikine QuicKit ELISA在血清和血浆中比品牌A具有更好的恢复能力。
Figure 5. R&D Systems TNF-alpha Quantikine® QuicKitTM ELISAs have superior recovery to Brand A in serum and plasma.
图 5.R&D Systems 的TNF-alpha Quantikine QuicKit ELISA在血清和血浆中比品牌A具有更好的恢复能力。
Quantikine QuicKit 比色夹心法ELISA试剂盒信息由R&D Systems为您提供，如您想了解更多关于Quantikine QuicKit 比色夹心法ELISA试剂盒报价、型号、参数等信息，欢迎来电或留言咨询。
注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途