Reversed-Phase Column with High Aromatic Selectivity for the Separation of Aromatic Compounds

Acclaim^®Phenyl-1 columns provide unique selectivity for aromatic compounds while maintaining sufficient hydrophobic interaction and aqueous compatibility for superior chromatographic performance.

Acclaim Phenyl-1 is based on covalent modification of high-purity, spherical, porous silica particles, with a specially designed silane ligand-bearing proprietary alkyl aromatic functionality. This novel column chemistry results in the following benefits.

• High aromatic selectivity

• High hydrophobic retention

• Unique and complementary selectivity

• Compatibility with highly aqueous mobile phase

• High efficiency and rugged packing

Acclaim Phenyl-1 columns are available in 3 μm particle sizes and 4.6, 3.0, and 2.1 mm diameters, with an average pore diameter of 120 Å. Acclaim Phenyl-1 columns may be used for LC/MS applications.

Applications

The Acclaim Phenyl-1 column has a unique bonded phase which has both enhanced aromatic selectivity and high hydrophobicity compared to other commercial phenyl-type phases, including biphenyl, diphenyl, C6-phenyl and C3-phenyl phases. It is also compatible with aqueous mobile phases. These capabilities make this column ideal for a broad range of applications including drug development and testing in which analytes commonly contain rings, conjugation, and ring substituents.

Separation of glucocorticosteroids

Separation of estrogens

Separation of fat-soluble vitamins

Analysis of soybean lecithin and egg lecithin

The National Institute of Standards and Technology (NIST) Standard Reference Material (SRM) 869a is useful for characterizing liquid chromatographic (LC) column selectivity for separation of PAHs.

These separations demonstrate that the Acclaim Phenyl-1 column shows different and complementary selectivity to other reversed-phase columns.