产地：MBI 

货号：EN0582

Applications

Primer removal from PCR mixtures:

– prior to PCR product sequencing (2),

– for one-tube "megaprimer" PCR mutagenesis (3).

Removal of single-stranded DNA containing a 3'-hydroxyl terminus from nucleic acid mixtures.

Assay for the presence of single-stranded DNA with a 3'-hydroxyl terminus (4).

Description

Exonuclease I (ExoI) degrades single-stranded DNA in a 3'=>5' direction, releasing deoxyribonucleoside 5'-monophosphates in a stepwise manner and leaving 5'-terminal dinucleotides intact, see Fig.1.

It does not cleave DNA strands with terminal 3'-OH groups blocked by phosphoryl or acetyl groups (1).