产地：MBI 

货号：EP0091

Applications

Rolling circle amplification (RCA) (6): generation of periodic DNA nanotemplates (7).

Multiple displacement amplification (MDA) (8).

Unbiased amplification of whole genome (WGA, see Fig.1):

– amplification of DNA for SNP (9) and STR (10) detection,

– cell-free amplification of DNA from single cells (11, 12),

– pathogenic organisms or metagenomes (13),

– amplification of DNA from filter paper blood spot samples (14).

DNA template preparation for sequencing.

Protein-primed DNA amplification (15).

In situ genotyping with padlock probes (17).

Recombination based-cloning (18).

Cell-free cloning of lethal DNA (19).

RNA-primed DNA amplification (16).

Description

phi29 DNA Polymerase is a highly processive polymerase (up to more than 70 kb) featuring strong strand displacement activity which allows for highly efficient isothermal DNA amplification (1). phi29 DNA Polymerase also possesses a 3'=>5' exonuclease (proofreading) activity acting preferentially on single-stranded DNA (2) or RNA (3). Therefore 3'-modified primers are highly recommended (4).