Successful amplification of GC-rich and complex targets
A specially engineered enzyme and optimized buffer system enable the Herculase II enzyme to amplify difficult targets with high yields using fast cycling conditions. Targets that contain as much as 84% GC content are easily amplified whereas other competitors’ enzymes failed or produced low yield. To enhance amplification of problematic or GC-rich templates, DMSO (provided in a separate tube) can be added (3–10% DMSO; titrated in 1% increments to find optimal performance with your targets).
