产地：MBI
货号：EP0161 Applications
Production of synthetic homo- and heteropolymers (1).
Homopolymeric tailing of linear duplex DNA with any type of 3'-OH terminus (2, 3).
Oligodeoxyribonucleotide and DNA labeling (2, 4-8).
5'-RACE (Rapid Amplification of cDNA Ends) (9).
In situ localization of apoptosis (10).
Description
Terminal Deoxynucleotidyl Transferase (TdT), a template-independent DNA polymerase, catalyzes the repetitive addition of deoxyribonucleotides to the 3'-OH of oligodeoxyribonucleotides and single-stranded and double-stranded DNA (1). TdT requires an oligonucleotide of at least three nucleotides to serve as a primer. With RNA as template TdT shows variable performance which strongly depends upon the tertiary structure of acceptor RNA 3'-end and the nature of nucleotide. Generally, it is lower than using DNA as a template.
EP0161 |
500 u (20 u/µl) |
0.40 ml |
EP0161 |
|
EP0162 |
2500 u (20 u/µl) |
2 x 1.00 ml |
EP0162 |
|
