产地：MBI 

货号：ER0231

Conditions for 100% Activity

1X Buffer Tango™:

33 mM Tris-acetate (pH 7.9 at 37°C), 10 mM Mg-acetate, 66 mM K-acetate and 0.1 mg/ml BSA.

Incubate at 37°C.

Storage Buffer

Eam1104I is supplied in:

10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/ml BSA and 50% (v/v) glycerol.

Ligation and Recleavage

After 50-fold overdigestion with Eam1104I, more than 95% of the DNA fragments can be ligated and recut.