20S Proteasome alpha6 antibody
Cat.#: S2830
Rabbit Polyclonal Antibody
Clone ID: polyclonal
Swiss Prot: P60900
Mol Weight: 27.4kDa
Size: 100ug

Description
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. A pseudogene has been identified on the Y chromosome. [provided by RefSeq]

Recommended Applications
WB, IHC, ICC

Applications and Recommended Dilution Factors
WB: 1:500-1:3000
IHC: 1:100-1:500,IF:1:100-1:200
ICC: 1:100-1:200

Species Reactivity
Human

Other species have not been tested

Products Data 

 

 
Sample (30ug of whole cell lysate)
A: 293T
B: A431
C: H1299
D: Hela
12% SDS PAGE
diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded H1299 xenograft , using 20S Proteasome alpha6 antibody at 1:100 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed A431, using 20S Proteasome alpha6 antibody at 1:200 dilution.

Specificity
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 57 and 244 of 20S Proteasome alpha6

Storage Condition and Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Purified by antigen-affinity chromatography.

Alternative Names
PSMA6 antibody, IOTA antibody, MGC22756 antibody, MGC2333 antibody, MGC23846 antibody, PROS27 antibody, p27K antibody, Proteasome subunit alpha type-6 antibody, Macropain iota chain antibody, Multicatalytic endopeptidase complex iota chain antibody, Proteasome iota chain antibody