AMPK alpha 1(PRKAA1)antibody
Cat.#: 1596-1
Rabbit Monoclonal Antibody
Clone ID: Y365
Swiss Prot: Q13131
Mol Weight: 63kDa
Size: 100ul

Description
The 5-AMP-activated protein kinase (AMPK), a member of the SNF1 (sucrose nonfermentor) kinase family (1) AMPK is a heterotrimeric protein comprising α (63 kDa), β (38 kDa) and γ (38 kDa) subunits. The alpha subunit is the catalytic subunit (which requires phosphorylation to be active) (2-3). Beta and gamma are noncatalytic subunits, although they have been found to interact with the active subunit in liver. AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (4). AMPK also appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways.

Recommended Applications
WB, IHC, IP

Applications and Recommended Dilution Factors
WB: 1:1,000-10,000
IHC: 1:100-250
IP: 1:50

Species Reactivity
Human, Mouse, Rat

Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only

Products Data 

 

A. Western blot analysis of anti-AMPK alpha-1 RabMAb (cat. # 1596-1), dilution 1:5,000. A: MCF-7 B: Hela
B. Immunohistochemical analysis of paraffin-embedded human cervical carcinoma using anti-AMPK alpha RabMAb (cat. # 1596-1).

Specificity
A synthetic peptide corresponding to residues near the C-term of human AMPK alpha-1 subunit was used as an immunogen

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
PRKAA1 antibody, AMPK antibody, AMPKa1 antibody, MGC33776 antibody, MGC57364 antibody, 5'-AMP-activated protein kinase catalytic subunit alpha-1 antibody, antibody

Description References
1. Mitchelhill, K. I., Stapleton, D., Gao, G., House, C., Michell, B., Katsis, F., Witters, L. A., and Kemp, B. E. (1994) J. Biol. Chem. 269, 2361-2364
2. Stapleton et al. J. Biol. Chem. 271:611-614
3. Hamilton et al. FEBES Lett. 500:163-168, 2001
4. Carling, D., Clarke, P. R., Zammit, V. A., and Hardie, D. G. (1989) Eur. J. Biochem. 186, 129-136

Product References
1. Abiola M, Favier M, Christodoulou-Vafeiadou E, et al, Activation of Wnt/β-Catenin Signaling Increases Insulin Sensitivity through a Reciprocal Regulation of Wnt10b and SREBP-1c in Skeletal Muscle Cells ,PLoS One 4 (2009) e8509
[Application: WB]