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| Catalogue Number | ANT-245 |
| Type | Mouse Anti Human Monoclonal Antibody. |
| Introduction | Vimentin expression in human malignant glioma cells depends on cellular density, algorithms of drug delivery and chemo/radio treatment. Vimentin and detyrosinated microtubules provide structural support for the extensive microtentacles observed in detached tumor cells and a mechanism to promote successful metastatic spread. Primary colorectal carcinomas display aberrant expression of vimentin, and have activated Notch and TGFbeta signaling pathways. Vimentin is a strong arterial substrate for transglutaminases. Transglutaminase-mediated vimentin dimerization results in a novel unifying pathway by which vasodilatory and remodeling responses may be regulated. Ablation of vimentin expression inhibits migration and invasion of colon and breast cancer cell lines. |
| Clone | hVimentin. |
| Immunogen | Purified Human Vimentin. |
| Ig Subclass | mouse IgG1. |
| Purification Method | Ion exchange column. |
| Protein concentration | 1mg/ml in PBS (after reconstitution). |
| Shipping Conditions | Antibody is shipped lyophilized at ambient temperature. |
| Storage Procedures | In lyophilized form, for long periods, store at 4°C in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20°C. |
| Reconstitution | Reconstitute with H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Stability / Shelf LifeTwo years lyophilized, one month in solution at 4°C. |
| Titer | For intra-cellular staining, use 5-10µl/106 cells. Wash twice with PBS/Azide before adding the appropriate amount of secondary fluorescent reagent. |
| Applications | Direct ELISA, Western Blot, Immuneprecipitation, immunohistochemistry. |
| Cross Reactivity | Vimentin antibody will recognize most mammalian Vimentin except mouse and rat. |
| Other Available Forms | This antibody is also available conjugated to biotin and FITC. |
| Usage | ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
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