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MouseAntiHumanCD14Biotinylated

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Catalogue Number ANT-252 Synonyms Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein

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耗材描述

Catalogue Number

ANT-252

Synonyms

Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein.

Type

Mouse Anti Human Monoclonal.

Introduction

CD14 (also known lipopolysaccharide (LPS) receptor) is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation. CD14 has been shown to bind apoptotic cells.

Clone

hCD4.

Immunogen

Purified human B Cells.

Ig Subclass

Mouse IgG1.

Purification Method

Ion exchange column.

Protein concentration

1mg/ml in PBS (after reconstitution).

Storage Procedures

Lyophilized: store at 4°C. After reconstitution, if not intended for use within a month, aliquot and store at -20°C.

Reconstitution

Reconstitute with of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Stability / Shelf LifeTwo years lyophilized, one month in solution at 4°C.

Antibody Properties

For staining, use 10 µl/106 cells.

Available Conjugates

This antibody is also available as purified antibody and conjugated to FITC.

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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MouseAntiHumanCD14Biotinylated