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MouseAntiHumanCD4

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品牌：Prospec
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Catalogue Number ANT-145 Synonyms gp55, HLA-2, L3 / T4, Ly-4, T cell antigen T4/LEU3, T4, sCD4, CD4mut. Type Mouse Anti

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耗材描述

Catalogue Number

ANT-145

Synonyms

gp55, HLA-2, L3 / T4, Ly-4, T cell antigen T4/LEU3, T4, sCD4, CD4mut.

Type

Mouse Anti Human Monoclonal.

Introduction

CD4 is a cell-surface glycoprotein found on the mature helper T cells and immature thymocytes, as well as on monocytes and macrophages. (Some cytotoxic T cells have CD4 protein as well.) Normally, about 65% of T cells in the blood are CD4+ (have CD4 protein protruding from their membrane). A mature T cell with either have CD4 or CD8, but not both. During one stage of development T cells develop CD4 and CD8 receptors, but they eventually are differentiated in the thymus and become more specialized.

Clone

hCD4.

Immunogen

Purified human PBL CD4+ T cells.

Ig Subclass

Mouse IgG2b.

Purification Method

Ion exchange column.

Protein concentration

1mg/ml in PBS (after reconstitution).

Storage Procedures

Lyophilized: store at 4oC. After reconstitution, if not intended for use within a month, aliquot and store at -20oC.

Reconstitution

Reconstitute with of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Stability / Shelf LifeTwo years lyophilized, one month in solution at 4oC.

Antibody Properties

Blocking and staining antibody. Blocks blinding of HIV to CD4. For staining, use 10 µl/106 cells. Titer for blocking T cell activation should be determined by the investigator.

Available Conjugates

This antibody is also available conjugated to biotin and FITC.

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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