Apolipoprotein A-II(ApoA-II)(APOA2)antibody
Cat.#: 2744-1
Rabbit Monoclonal Antibody
Clone ID: EPR2913
Swiss Prot: P02652
Mol Weight: 11kDa
Size: 100ul

Description
Apolipoprotein A II is the second most abundant protein of the high density lipoprotein particles, accounting for 20% of protein mass of HDL. The concentration of ApoA-I and ApoA-II is inversely correlated with the frequency of heart disease in human (1). In studies with mice and humans, ApoA-II levels have been associated with increased susceptibility to atherosclerosis, increased free fatty acid levels, increased body fat, and increased insulin resistance (2). ApoA-II consists of three potential amphipathic helices of 17 residues each, which contribute to the lipid-binding properties of this apolipoprotein (3). Currently, the best characterized activity of apoA-II is its potent antagonism of the anti-atherogenic and anti-inflammatory activities of ApoA-I, probably due to its competition with the latter for lipid acyl side chains of HDL (1).

Recommended Applications
WB, ICC, FC

Applications and Recommended Dilution Factors
WB: 1:1,000 - 5,000
ICC: 1:100 - 250
FC: 1:50

Species Reactivity
Human

Products Data 

 

Western blot analysis on human plasma lysates using anti-Apolipoprotein A-II (ApoA-II) RabMAb (cat. #2744-1).
Immunofluorescent staining of HEPG2 cells using anti-ApoAII RabMAb (cat. #2744-1).

Specificity
A synthetic peptide corresponding to residues in human Apolipoprotein A-II (ApoA-II) was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
APOA2 antibody, Apo-AII antibody, ApoA-II antibody, apoAII antibody, Apolipoprotein A-II(1-76) antibody, Apolipoprotein A2 antibody

Description References
1. Kumar et al. Biochemistry. 41 (39):11681-11691, 2002 2. Castellani and Lusis. Arterioscler. Thromb. Vasc. Biol. 21:1870-1872, 2001 3. Benetollo et al. Eur. J. Biochem. 242 :657-664, 1996