4-1BBL(TNFSF9)antibody
Cat.#: 2305-1
Rabbit Monoclonal Antibody
Clone ID: EPR1172Y
Swiss Prot: P41273
Mol Weight: 26kDa
Size: 100ul

Description
4-1BB was originally described as a cDNA expressed by activated murine T cells and subsequently demonstrated to encode a member of the tumor necrosis factor receptor family of integral membrane proteins. Recently, a murine ligand for 4-1BB (mu4-1BB-L) was cloned and demonstrated to be a member of an emerging family of ligands with structural homology to tumor necrosis factor. Both monoclonal antibody to hu4-1BB and cells transfected with hu4-1BB-L induced a strong proliferative response in mitogen co-stimulated primary T cells. In contrast, ligation of 4-1BB on T cell clones enhanced activation-induced cell death when triggered by engagement of the TCR/CD3 complex. (1). To reject tumors, T cells must overcome poor tumor immunogenicity and an adverse tumor microenvironment. It has been shown that primary human T cells expressing CD80 and 4-1BBL vigorously respond to tumor cells lacking costimulatory ligands and provoke potent rejection of large, systemic tumors in immunodeficient mice (2).

Recommended Applications
WB, IHC, ICC, IP

Applications and Recommended Dilution Factors
WB: 1:500
IHC: 1:100
ICC: 1:100
IP: 1:40

Species Reactivity
Human

Cross reactivity determined by western blot only.

Products Data

 
A. Western blot analysis on 293T cell lysate using anti-4-1BBL RabMAb (cat. # 2305-1), dilution 1:2000.

Specificity
A synthetic peptide corresponding to residues near the N-terminus of human 4-1BBL was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
TNFSF9 antibody, 4-1BB-L antibody, CD137L antibody, Tumor necrosis factor ligand superfamily member 9 antibody, 4-1BB ligand antibody

Description References
1. Alderson MR, et al. Eur J Immunol 24(9):2219-27, 1994
2. Steohan MT, et al. Nat Med 13(12):1440-9, 2007