Acclaim 300 C18 蛋白质和羧氨酸色谱柱
  蛋白质和羧氨酸的高分辨率分离
High-Resolution Separation of Proteins and Peptides

概要:
The Acclaim^® 300 features 3 µm silica particles for rapid analysis of complex protein digests. The Acclaim 300’s stable bonding make these columns compatible with a wide variety of applications, including LC/MS methods.

• Designed for high-resolution peptide mapping and protein separations
• High-efficiency 3 µm spherical silica substrate
• High-performance bonding chemistry on 300 Å pore silica
• Application tested for suitablility in peptide mapping
• Reproducible for dependable results
• LC/MS compatible

Manufacture of these columns starts with ultrapure silica that contains minimal concentrations of metal contaminants, minimizing the tailing effects of residual, exposed silanol groups. Exhaustive bonding and end-capping techniques result in stable bonding and columns that exhibit predictable reversed-phase separations with minimal secondary interactions. 

The use of a 3 µm silica particle reduces limits for diffusion of the analytes into the stationary phase, resulting in fast, high-resolution separations. Compared to 5 µm column packings, the smaller particles support increased flow rates and shallower gradients on shorter columns, for faster separations. Dionex tests each bonded silica lot for peptide selectivity to ensure reproducible performance.

规格:

Acclaim PepMap for TFA-Free Peptide Mapping  Specifications
Column type	Nano	Capillary	Micro
Column i.d.	75 µm	300 µm	1.0 mm
Length	50, 150, 250, and 500 mm	50, 150, and 250 mm	50, 150, and 250 mm
Recommended Flow Rate	200 nL/min	4 µL/min	40 µL/min
Packing Material	C18, 100 Å,3 µm	C18, 100 Å,3 µm	C18, 100 Å,3 µm
	C18, 300 Å,5 µm	C18, 300 Å,5 µm	C18, 300 Å,5 µm
	C8, 100 Å,3 µm	C8, 100 Å,3 µm	C8, 100 Å,3 µm
	C8, 100 Å,5 µm	C8, 100 Å,5 µm	C8, 100 Å,5 µm
	C4, 300 Å,5 µm	C4, 300 Å,5 µm	C4, 300 Å,5 µm
Column	Fused-silica	Glass Lined Tubing	Stainless steel
Column Inlet	1/16-in.PEEK sleeve adapter(provided)nanoViper (optional)	1/16-in.universal fittingnano Viper (optional) 50µm i.d. PEEK sheathed fused silica with nanoViper fittings	1/16-in.PEEK tubingnano Viper (optional) 75µm i.d. PEEK sheathed fused silica with nanoViper fittings
Column Outlet	Fused-silica 25-µm i.d./ 280-µm o.d.	Fused-silica 75-µm i.d./ 280-µm o.d	1/16-in.PEEK tubing nanoViper (optional) 75µm i.d. PEEK sheathed fused silica with nanoViper fittings
MS Outlet	1-m Length

订购信息：
The standard particle size is nominally 3 µm. Analytical columns are available in 2.1, 3.0, and 4.6 mm dimeters; standard lengths are 50 and 150 mm. Capillary formats are available in 75 and 300 µm, 1.0 mm, and custom diameters, and in 50, 150, or 250 mm lengths. Guard columns in both 2.0 and 4.3 mm sizes are recommended to protect analytical columns.

Analytical Columns
Acclaim 300, C18, 3 µm Analytical (2.1 x 50 mm)	60263
Acclaim 300, C18, 3 µm Analytical (2.1 x 150 mm)	60264
Acclaim 300, C18, 3 µm (3.0 x 150 mm)	63684
Acclaim 300, C18, 3 µm Analytical (4.6 x 50 mm)	60265
Acclaim 300, C18, 3 µm Analytical (4.6 x 150 mm)	60266
Guard Columns
Acclaim 300,C18, 3µm, Guard Cartridges, (2.1 x 10mm), 2 ea; requires holder 069580	69690
PROD,COL,ACCLAIM,300,C18,3UM,4.6X10MM,GRD,2EA	69697
Hardware
Acclaim SST Guard Cartridge Holder V-1	59456
Guard to Analytical Column Coupler	59457
Guard Kit (Holder and Coupler)	59526
Acclaim SST Guard Cartridge Holder V-2	69580
Acclaim Guard Kit (Holder and coupler) V-2	69707
Guard to Analytical Column Coupler V-2	74188
Micro and Nano Columns
Acclaim 300, C18, 3 µm, 300 Å, 75 µm i.d. x 5 cm	162223
Acclaim 300, C18, 3 µm, 300 Å, 75 µm i.d. x 15 cm	162224
Acclaim 300, C18, 3 µm, 300 Å, 300 µm i.d. x 5 cm	162221
Acclaim 300, C18, 3 µm, 300 Å, 300 µm i.d. x 15 cm	162222
Acclaim 120, C18, 3 µm, 120 Å, 1.0 mm i.d. x 5 cm	162219
Acclaim 300, C18, 3 µm, 300 Å, 1.0 mm i.d. x 15 cm	162220

应用谱图：