Application
The antibody recognizes the FLAG epitope located on FLAG-tagged fusion proteins at the N-terminus or C-terminus, applying dot blot, immunoblotting, immunoprecipitation and immunocytochemistry assays.
Browse additional application references in our FLAG® Literature portal.
Features and Benefits
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General description
The rabbit Anti-FLAG polyclonal affinity antibody ANTI-FLAG recognizes the FLAG epitope located on FLAG fusion proteins. This antibody reacts with N-terminal, N-terminal-Met, and C-terminal FLAG fusion proteins.
Immunogen
FLAG; peptide sequence DYKDDDDK
Preparation Note
Purified by affinity chromatography on a column bearing the immunizing peptide.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide
法律信息
FLAG is a registered trademark of Sigma-Aldrich Co. LLC