3-氨基-9-乙基咔唑
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分子式 C14H14N2 分子量210.28 Beilstein号 22(4)4936 EC号 MDL号 MFCD00004964 PubChem编号 属性 熔点 98°C-100°C 存贮条件 储存温度2-8℃ 密度 1.1700 //d
货号 | CAS号 | 价格 | 操作 |
---|---|---|---|
A105545-25g | 132-32-1 | ¥499.00 | 询底价 |
A105545-5g | 132-32-1 | ¥149.00 | 询底价 |
耗材描述
- 分子式 C14H14N2
- 分子量210.28
- Beilstein号 22(4)4936
- EC号
- MDL号 MFCD00004964
- PubChem编号
属性
熔点 | 98°C-100°C |
存贮条件 | 储存温度2-8℃ |
密度 | 1.1700 |
描述
应用 |
过氧化物酶底物. Usage Prepare samples and incubate with antibodies and HRP-labeled probes, follow with all necessary wash steps. ? Prepare 1X AEC Substrate Buffer (dilute AEC Substrate Buffer, 10X;). ? Dilute one part AEC, 50X with 50 parts 1X AEC Substrate Buffer to make 1X AEC Substrate Solution. Mix well. ? Cover tissue sections with 1X AEC Substrate Solution and incubate 10-30 min. at RT. ? Rinse thoroughly with distilled water. If desired, follow with an appropriate aqueous-based counterstain. ? Mount tissue samples with an aqueous-based mounting medium (e.g., Crystal Mounting Medium; ) ? Unused AEC solution can be discarded according to city, county, state, province or country’s regulations |
产品介绍 | AEC, 50X (3-amino-9-ethylcarbazole); for HRP-based detection systems; provides red chromogen depositions. Precipitable peroxidase substrate for immunohistochemical (IHC) staining techniques. In the presence of horseradish peroxidase (HRP), produces a water-insoluble red oxidation product. Although not as sensitive as diaminobenzidine (DAB, 50X;, the red deposit formed at the sites of AEC oxidation make this substrate useful for double labeling. Aqueous-based mounting medium and counterstains must be used for IHC applications since oxidized AEC is soluble in alcohols and xylene (e.g., Crystal Mounting Medium; ). Use with AEC Substrate Buffer . |
生化机理 | 3-氨基-9-乙基咔唑是一种过氧化酶底物,适用于免疫印迹和免疫组织化学染色程序。该底物能产生一种不溶性红色的末端产物。肉眼或可见光下就可观察。 |
别名 | AEC, 50X ;3-氨基-N-乙基咔唑;AEC, 50X |
Reaxys-RN | 174969 |
标识符号 | GHS06, GHS08 |
信号词 | Danger |
危害声明 | H301,H350 |
警示性声明 | P201,P301+P310,P308+P313 |
个人防护设备 | Eyeshields, Faceshields, full-face particle respirator type N100 (US), Gloves, respirator cartridge type N100 (US), type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges |
RTECS | FE3590000 |
风险声明 (欧洲) | R25;R36/37/38;R40 |
安全声明(欧洲) | S26;S36/37/39 |
危害码(欧洲) | T |
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