Platinum® Pfx DNA 聚合酶

极高保真度的热稳定 PCR 酶

1.保真度较 Taq DNA 聚合酶高 26 倍
2.扩增片段长达 12 kb
3.在室温下建立反应体系

Platinum® Pfx DNA 聚合酶 是高保真 PCR 应用中 DNA 片段扩增的理想选择。高保真度由专有的酶制剂提供，该酶制剂含有来自热球菌属KOD的重组DNA聚合酶，具有校对（3'→5'核酸外切酶）活性。Platinum®类抗体技术提供了一种简单、自动、热启动的方法，可提高 PCR 特异性（见图）。PCRx 增强剂解决方案可实现更高的引物特异性，更广泛的镁浓度余量，更广泛的退火温度参数，并可改善Platinum® Pfx DNA聚合酶的热稳定性。PCRx 增强剂解决方案还有助于优化有问题和/或富含 GC 的模板的 PCR。Platinum® Pfx DNA 聚合酶提供：

Platinum® Pfx DNA Polymerase is ideal for amplification of DNA fragments for high-fidelity PCR applications. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading (3´→5´ exonuclease) activity. Platinum® antibody technology provides a simple, automatic, hot-start method that improves PCR specificity (see figure). PCRx Enhancer Solution is included for higher primer specificity, broader magnesium concentration allowances, broader annealing temperature parameters, and improved thermostability of Platinum® Pfx DNA Polymerase. The PCRx Enhancer Solution also helps optimize PCR of problematic and/or GC-rich templates. Platinum® Pfx DNA Polymerase provides:

1.保真度较 Taq DNA 聚合酶高 26 倍
2.扩增片段长达 12 kb
3.在室温下建立反应体系

从复杂的基因组、病毒和质粒模板中扩增 DNA;和 RT-PCR。Platinum® Pfx DNA 聚合酶在需要非常高的保真度时特别有效。

• 26 times higher fidelity than Taq DNA polymerase
• Amplification of fragments up to 12 kb
• Room temperature reaction assemblyApplications
Amplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCR. Platinum® Pfx DNA Polymerase is especially effective when very high levels of fidelity are required.

单元定义
一个单元指：在74°C下30分钟内将10毫摩尔脱氧核糖核苷酸掺入酸性可沉淀材料中。

Unit definition
One unit incorporates 10 nmoles of deoxyribonucleotide into acid-precipitable material in 30 min at 74°C.