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CAS No. : 94885-02-6
MCE 国际站:C6 NBD Ceramide
产品活性:C6 NBD Ceramide 是一种具有细胞膜渗透性的高尔基体荧光探针 (Ex=466 nm, Em=536 nm)。C6 NBD Ceramide 能快速便捷地在活体细胞和固定细胞中对高尔基体进行绿色荧光标记,可用于观察活细胞中高尔基体形态的变化。C6 NBD Ceramide 能被代谢为荧光鞘磷脂和葡糖基甘油酰胺,可用于鞘脂转运和代谢机制的研究。
研究领域:Others
作用靶点:Fluorescent Dye
In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Labeling of Golgi apparatus:
1. Rinse cells (on glass cover slips) by HMEM, then fix cells in glutaraldehyde or paraformaldehyde for 5-10 min (25℃).
2. Wash fixed cells in a balanced salt solution (such as HCMF).
3. Transfer culture dishes containing the glass cover slips to an ice-water bath and incubate with freshly prepared NaBH4 for 3×5 min (for glutaraldehyde-fixed cells).
4. Rinse cells several times (20-30 min) in cold HCMF, warm to 25℃, then incubate with C6 NBD-ceramide/BSA for 60 min.
5. Wash fixed cells by HCMF, and incubate with 10% fetal calf serum or 2 mg/mL BSA (30-90 min; 25℃) to back-exchange excess C6 NBD-ceramide from the preparation and enhance the staining of the Golgi apparatus.
6. The glass cover slips mounted on depression slides and observe by fluorescence microscope (Ex=466 nm, Em=536 nm).
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