Hoechst 33258 analog 产品活性：Hoechst 33258 analog 是 Hoechst 系列的标记染料。Hoechst 系列是活细胞核标记染料。Hoechst 通过结合 DNA 双链中的小沟而结合核酸。Hoechst 倾向于结合富含 A/T 的 DNA 链。同时，富含 A/T 的双链 DNA 使荧光强度显著增强。Hoechst 可穿过细胞膜，结合活细胞或固定细胞。Hoechst 染料的荧光强度随着溶液 pH 升高而增强。 产品来源： https://www.medchemexpress.cn/Hoechst-33258-analog.html 研究领域：Cell Cycle/DNA Damage 作用靶点：DNA Stain In Vitro: General Protocol Preparation of Hoechst working solution 1.1 Preparation of the stock solution Dissolve 10 mg of in 5 mL DMSO Note: It is recommended to store the stock solution at 4℃ or -20℃ away from light and avoid repetitive freeze-thaw cycles. 1.2 Preparation of Hoechst working solution Dilute the stock solution in serum-free cell culture medium or PBS to obtain final concentration 10 μg/mL Hoechst working solution. Note: Please adjust the concentration of Hoechst working solution according to the actual situation. 1.Cell staining 2.1 Suspension cells（6-well plate） a. Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. The cell density is 1×106/mL. b. Add 1 mL of working solution, and then incubate at room temperature for 3-10 minutes. c. Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant. d. Wash twice with PBS, 5 minutes each time. e. Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry. 2.2 Adherent cells a. Culture adherent cells on sterile coverslips. b. Remove the coverslip from the medium and aspirate excess medium. c. Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 3-10 minutes. d. Wash twice with medium, 5 minutes each time.Observation by fluorescence microscopy or flow cytometry. Precautions 1. Please adjust the concentration of Hoechst working solution according to the actual situation. 2. This product is for R&D use only, not for drug, household, or other uses. 3. For your safety and health, please wear a lab coat and disposable gloves to operate. In Vivo: Hoechst dyes are cell-permeable and can bind to DNA in live or fixed cells. 相关产品：DAPI dihydrochloride | Acridine Orange hydrochloride | Ethidium bromide | DFHBI-1T | Pyronin Y | HBC | CY5-N3 | Biotin-aniline | Cy5 maleimide | 7-Aminoactinomycin D | 2-Aminopurine | CellTracker Blue CMF2HC Dye | Cy5.5-SE (DIPEA) | LDS-751 | 5(6)-TAMRA SE | 6-TAMRA | 6-TAMRA-SE | Cyanine 5 Tyramide | Ethidium homodimer | 5(6)-ROX | Methyl Green | 6-Carboxy-X-rhodamine, succinimidyl ester | DBCO-PEG4-TAMRA | TO-PRO 1 | Biotin-11-dCTP | Cy3-dCTP | Methyl Green zinc chloride | Pyronin B | Biotin-16-dUTP trisodium 品牌介绍: MCE (MedChemExpress) 拥有数百种全球独家化合物，我们致力于为全球科研客户提供最新最全的高品质小分子活性化合物；10,000 多种高选择性抑制剂、激动剂涉及各热门信号通路及疾病领域；设有专业的实验中心和严格的质控、验证体系；提供 LC/MS、NMR、HPLC、手性分析、元素分析等各项质检报告，确保产品的高纯度、高品质；产品的生物活性多经各国客户实验验证；Nature, Cell, Science 等多种顶级期刊及制药专利收录了MCE客户的科研成果；专业团队跟踪最新的制药及生命科学研究进展，为您提供全球最新的活性化合物；与世界各大制药公司及知名科研机构建立了长期的合作。