MQA-P 产品活性：MQA-P 是一种多功能近红外 (NIR) 荧光探针，可同时检测线粒体内的 ONOO-、粘度和极性。MQA-P 对 ONOO- 表现出显着的响应，λem=645 nm；并且在 λem>704 nm 的 NIR 通道中对粘度/极性高度敏感。MQA-P 具有激发态分子内电荷转移 (ESICT) 特性，通过将 N,N-二甲基氨基设计为电子供体和将喹啉阳离子单元设计为电子受体，其对极性高度敏感。MQA-P 通过双通道图像在体外和体内用于铁死亡或癌症诊断。 产品来源： https://www.medchemexpress.cn/mqa-p.html 研究领域：Others 作用靶点：Others In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 1. MQA-P is dissolved in dimethyl sulfoxide (DMSO) to prepare a stock solution (1.0 mM). 2. For imaging of ONOO- in live cells. HeLa cells are incubated with MQA-P (5 μM) for 30 min as control; pretreated with SIN-1 (HY-126849; 100 μM) for 30 min and then incubated with MQA-P (5 μM) for another 30 min. The fluorescence images are obtained on a confocal laser scanning microscope with a green channel (λex= 405nm, λem= 550-670 nm). 3. For imaging of viscosity in live cells. HeLa cells were incubated with MQA-P (5 μM) for 30 min as control; pretreated with Monensin (HY-N4302; 10 μM) for 30 min and then incubated with MQA-P (5 μM) for another 30min. The fluorescence images are obtained on a confocal laser scanning microscope with a red channel (λex= 561 nm, λem= 680-750 nm). 4. For dual-channel imaging of ONOO-, viscosity and polarity during ferroptosis. HeLa cells are incubated with MQA-P (5 μM) for 30 min as control; pretreated with Erastin (HY-15763; 50 μM) for 30 min and then incubated with MQA-P (5 μM) for another 30 min. The fluorescence images are obtained on a confocal laser scanning microscope with a green channel (λex= 405nm, λem= 550-670 nm) for ONOO- and a red channel (λex= 561 nm, λem= 680-750 nm) for viscosity and polarity. In Vivo: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 1. For tissue slices imaging, the normal organs (including heart, liver, spleen, lung, and kidney) and tumor are isolated from the mice, then sectioned as 5 μm thicknesses, respectively. 2. These slices are incubated with MQA-P (20 μM) for 30 min, then washed with PBS (pH 7.4) three times, and finally subjected to in vivo imaging using a confocal laser scanning microscope with a green channel (λex=405nm, λem=550-670 nm) for ONOO- and a red channel(λex=561 nm, λem=680-750 nm) for viscosity and polarity, respectively. 相关产品：Digitonin | 5-Ph-IAA | FLAG peptide | 3X FLAG peptide | TCEP hydrochloride | Monocrotaline | Tocofersolan | Pronase E (Activity ≥ 7000 U/g) | DPPH | DNase I, Bovine pancreas | NMDI14 | O-Propargyl-Puromycin | Human IgG1 kappa, Isotype Control | Phytohemagglutinin | Hyaluronidase | Biotinyl tyramide | MYLS22 | Pertussis Toxin | N,N-Dimethylformamide | Kifunensine | OVA Peptide (323-339) | L-Tryptophan | 6-Aminonicotinamide | Lauryl maltose neopentyl glycol | Alkyne-phenol | Potassium oxonate | OVA Peptide(257-264) TFA | CHAPS | Teriparatide 品牌介绍: MCE (MedChemExpress) 拥有数百种全球独家化合物，我们致力于为全球科研客户提供最新最全的高品质小分子活性化合物；10,000 多种高选择性抑制剂、激动剂涉及各热门信号通路及疾病领域；设有专业的实验中心和严格的质控、验证体系；提供 LC/MS、NMR、HPLC、手性分析、元素分析等各项质检报告，确保产品的高纯度、高品质；产品的生物活性多经各国客户实验验证；Nature, Cell, Science 等多种顶级期刊及制药专利收录了MCE客户的科研成果；专业团队跟踪最新的制药及生命科学研究进展，为您提供全球最新的活性化合物；与世界各大制药公司及知名科研机构建立了长期的合作。