中文名:1,4-二硫苏糖醇
英文名:1,4-Dithiothreitol (DTT)
中文别名:1,4-二巯基苏糖醇, 克莱兰氏试剂|DL-1,4-二硫苏糖醇|二硫代苏糖醇|DL-二硫苏糖醇
英文别名:Dithiothreitol|DL-1,4-Dithiothreitol|dl-Dithiothreitol|3483-12-3|(2S,3S)-1,4-DIMERCAPTOBUTANE-2,3-DIOL|1,4-Dithiothreitol|D-1,4-Dithiothreitol|D-Dithiothreitol|27565-41-9|D-Dtt|1,4-Dithio-dl-threitol|threo-1,4-Dimercapto-2,3-butanediol|(2S,3S)-1,4-disulfa
纯度:≥97%
货号:D265376
Cas号:3483-12-3
存储温度:2-8°C储存,避光,充氩
产品介绍:
Overview
Reducing agent
Application
Use 1,4-Dithiothreitol primarily to protect free SH-groups from oxidation. Use it routinely in all work with enzymes and proteins during enzyme measurement and the characterization of proteins.
• As one of the reduction and alkylation reactants of the main allergen αs1-casein in milk.
• As a component of sperm membrane removal and reactivation medium.
•Because mercaptans can effectively protect the active sites of biocatalysts and maintain the stability of enzymes.
• As a reducing agent to test the substitution of N-alkaline maleic substitution and the reaction of excess radicals.
Before SDS-PAGE, DTT can effectively reduce protein disulfide bonds in the sample buffer. Both the irritation and toxicity of DTT are reduced by 2-mercaptoethanol. Usually, the required concentration of DTT (100mM) is used. It uses one-seventh of the concentration of 2-cholesterol-based ethanol (5% v/v, 700 mM).
Benefits
1.Rely on the higher stability of DTT in comparison to 2-mercaptoethanol
in aqueous solution
2.Experience more convenience and a less disagreeable odor
3.Benefit from the reduced tendency to oxidize in air.
Specification
Appearance: White to yellowish crystallizate
DTT (with Ellman's reagent): ≥97%
Thin layer chromatography: Chromatographically homogeneous, corresponds to reference
Stability: At +2 to +8°C within specification range for 24 months. Store dry. Protect from light.
查看阿拉丁官网此产品相关对应页面:https://www.aladdin-e.com/zh_cn/D265376.html