In Vitro: HJ-PI01 (0-3200 nmol/L; 48 hours) dose-dependently inhibits MDA-MB-231 cell growth and shows a substantial improvement compared with chlorpromazine and PI003.HJ-PI01 (100-400 nmol/L; 24 hours) shows weak toxicity to normal non-cancer cells.HJ-PI01 (300 nmol/L; 24 hours) induces autophagic cell death of MDA-MB-231 cells.HJ-PI01 (300 nmol/L; 24 hours) induces apoptotic cell death in MDA-MB-231 cells.HJ-PI01 (300 and 460 nmol/L; 12-48 hours) affects the expression levels of autophagy and apoptosis-related proteins.
In Vivo: HJ-PI01 (40 mg/kg; oral administration, once daily for 10 days) inhibits tumor growth in MDA-MB-231 xenograft mice.