BODIPY 493/503 methyl bromide | MedChemExpress (MCE)
品牌 | 厂商性质 | 产地 | 货期 | 价格 |
---|---|---|---|---|
MedChemExpress (MCE) | 生产商 | 美国 | 现货 | 440 |
性能特点
BODIPY493/503 methyl bromide 是一种 BODIPY 染料。BODIPY 染料是一种具有强紫外吸收能力的小分子染料,其荧光峰比较尖锐,量子产率高。其对环境的极性和 p
规格 | CAS号 | 价格 | 操作 |
---|---|---|---|
1 mg | 216434-81-0 | ¥440.00 | 询底价 |
MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务。
BODIPY 493/503 methyl bromide
CAS No. : 216434-81-0
MCE 国际站:BODIPY 493/503 methyl bromide
产品活性:BODIPY493/503 methyl bromide 是一种 BODIPY 染料。BODIPY 染料是一种具有强紫外吸收能力的小分子染料,其荧光峰比较尖锐,量子产率高。其对环境的极性和 pH 值相对不敏感,因此,在不同生理条件下相对稳定。由于其结构的非对称性,BODIPY 具有多种衍生结构产物。BODIPY 脂滴类染料可以很好的穿过细胞膜进入细胞内部,通过在细胞内的极性脂类上定位以进行特异性染色,因此,该染料可用于标记活细胞和固定细胞。最大激发/发射波长:493/503 nm。
研究领域:Others
作用靶点:Fluorescent Dye
In Vitro: General Protocol
1. Preparation of BODIPY493/503 methyl bromide working solution
1.1 Preparation of the stock solution
Dissolve 1 mg BODIPY493/503 methyl bromide in 293.3 μL DMSO to obtain 10 mM of stock solution.
Note: It is recommended to store the stock solution at -20°C or -80°C, keep away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of BODIPY493/503 methyl bromide working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-10 μM of working solution.
Note: Please adjust the concentration of BODIPY493/503 methyl bromide working solution according to the actual situation.
2. Cell staining
2.1 Suspension cells (6-well plate)
a. Centrifuge at 1000 g at 4°C for 3-5 min and then discard the supernatant. Wash twice with PBS, 5 min each time.The cell density is 1×106/mL.
b. Add 1 mL of working solution, and then incubate at room temperature for 5-30 min.
c.Centrifuge at 400 g at 4°C for 3-4 min and then discard the supernatant.
d. Wash twice with PBS, 5 min each time.
e. Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
2.2 Adherent cells
a. Culture adherent cells on sterile coverslips.
b. Remove the coverslip from the medium and aspirate excess medium.
c. Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 5-30 min.
d. Wash twice with medium, 5 min each time. Observation by fluorescence microscopy or flow cytometry.
Storage
-20°C, 1 year; Protect from light.
Precautions
1. Please adjust the concentration of BODIPY493/503 methyl bromide working solution according to the actual situation.
2. Experiments suggest a positive control, incubate the control group cells with 30 μM oleic acid for 8 h and then perform subsequent experiments.
3. This product is only for R&D use, not for drug, household, or others.
4. For your safety and health, please wear a lab coat and disposable gloves to operate.
相关产品:MitoSOX Red | BODIPY 493/503 | D-Luciferin potassium | Nile Red | FITC | JC-1 | Propidium Iodide | DiR | CFDA-SE | FITC-Dextran (MW 4000) | Dihydroethidium | 2-NBDG | Calcein-AM | DiI | Thiazolyl Blue | Cy5.5 | CY5 | TMRE | Fluo-4 AM | Indocyanine green | PKH 26 | Acridine Orange hydrochloride | DiD perchlorate | FM4-64 | BODIPY 581/591 C11 | CY5-SE | DiO
热门产品线:重组蛋白 | 药物筛选 | 天然产物 | 荧光染料 | PROTAC | 同位素标记物
Trending products:Recombinant Proteins | Bioactive Screening Libraries | Natural Products | Dye Reagents | PROTAC | Isotope-Labeled Compounds
品牌介绍:
• MCE (MedChemExpress) 拥有200 多种全球独家化合物库,我们致力于为全球科研客户提供前沿最全的高品质小分子活性化合物;
• 50,000 多种高选择性抑制剂、激动剂涉及各热门信号通路及疾病领域;
• 产品种类涵盖各种重组蛋白,多肽,常用试剂盒 ,更有 PROTAC、ADC 等特色产品,广泛应用于新药研发、生命科学等科研项目;
• 提供虚拟筛选,离子通道筛选,代谢组学分析检测分析,药物筛选等专业技术服务;
• 设有专业的实验中心和严格的质控、验证体系;
• 提供 LC/MS、NMR、HPLC、手性分析、元素分析等各项质检报告,确保产品的高纯度、高品质;
• 产品的生物活性多经各国客户实验验证;
• Nature, Cell, Science 等多种顶级期刊及制药专利收录了MCE客户的科研成果;
• 专业团队跟踪最新的制药及生命科学研究进展,为您提供全球最新的活性化合物;
• 与世界各大制药公司及知名科研机构建立了长期的合作。
![MCE Hotline: 4008203792 | 中国现货 - 全球文献引用 - 高纯度高品质 - 全方位技术支持](https://file.medchemexpress.cn/web-groups/product-type/7.jpg)
BODIPY 493/503 methyl bromide | MedChemExpress (MCE)信息由MedChemExpress (MCE)为您提供,如您想了解更多关于BODIPY 493/503 methyl bromide | MedChemExpress (MCE)报价、型号、参数等信息,欢迎来电或留言咨询。
注:该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案,不可用于临床诊断或治疗等相关用途