EP0501	100 u (2.5 u/µl)	0.60 ml	0.60 ml	0.60 ml	EP0501
EP0502	500 u (2.5 u/µl)	2 x 1.25 ml	2 x 1.25 ml	2 x 1.25 ml	EP0502

Pfu DNA Polymerase (native)

Catalog#	Size, concentration	Supplied with:			Certificate of Analysis
		10X Pfu Buffer	10X Pfu Buffer with MgSO4	25 mM MgSO4
EP0571	100 u (2.5 u/µl)	0.60 ml	0.60 ml	0.60 ml	EP0571
EP0572	500 u (2.5 u/µl)	2 x 1.25 ml	2 x 1.25 ml	2 x 1.25 ml	EP0572

产地：MBI 

货号：EP0502

Applications

High fidelity PCR.

Generation of PCR products for cloning and expression.

RT-PCR for cDNA cloning and expression.

Blunt-end PCR cloning (1).

Site-directed mutagenesis.

Description

Pfu DNA Polymerase is a highly thermostable DNA polymerase from the hyperthermophilic archaeum Pyrococcus furiosus. The enzyme catalyzes the template-dependent polymerization of nucleotides into duplex DNA in the 5'=>3' direction. Pfu DNA Polymerase also exhibits 3'=>5' exonuclease (proofreading) activity, that enables the polymerase to correct nucleotide incorporation errors. It has no 5'=>3' exonuclease activity.