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Immunogen
N-terminal synthetic decapeptide of α-smooth muscle actin.
Other Notes
To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide, as a preservative.
Application
Immunocytochemistry was performed on smooth muscle cells from bovine aortas using the monoclonal anti-ACTA2 antibody. Cells were first grown on glass cover slips and fixed in 50% acetone/EtOH for 10 minutes at 4 degrees.
Monoclonal Anti-Actin, α-Smooth Muscle - FITC antibody is suitable for:
• immunohistochemical (IHC) staining of heart, lung, kidney, and liver of mice to examine the PHD2 deficiency in internal organs[2]
• immunostaining to analyze vascular smooth muscle cells/pericytes development in platelet-derived growth factor (PDGFR) and PDGFR- β-/- embryos[3]
• immunofluorescent analysis of implanted cells, frozen tissue samples to study the similar levels of expression of SMaA and SM-MHC as established primary SMC lines[4]
• western blots of lens epithelial cells to determine the distribution of α-SMA expression in lens epithelial cell[7]
Paraffin embedded sections of rat testis tissue grafts were immunohistochemically stained with mouse monoclonal anti-smooth muscle actin.
IHC analysis of x-gal stained muouse cardiac tissue was performed using the primary antibody, mouse monoclonal anti-smooth muscle actin to identify myofibroblasts.
Biochem/physiol Actions
Actin, a conserved eukaryotic protein, is the major cytoskeletal component which actively participates in cell motility. In mammals and birds, actin is expressed as six different isoforms.[5][6] α-Smooth muscle actin, a smooth muscle cell-specific protein, is also known as aortic smooth muscle or α smooth muscle actin (α-SMA, SMactin, α-SM-actin, ASMA). It belongs to actin family, which are highly conserved proteins that play a role in cell motility, structure and integrity. It is predominantly found in cells of the smooth muscle lineages. It has been reported that α-smooth muscle actin was also found in certain non-muscle cells, in particular fibroblasts i.e. myofibroblasts.[1]
General description
The antibody (also known as anti-α-Sm-1) is specific for the single isoform of α-smooth muscle actin. It reacts specifically with α-smooth muscle actin in immunoblotting assays and labels smooth muscle cells in frozen or formalin-fixed, paraffin-embedded tissue sections.
性质
Related Categories | AC-AC, Antibodies against Proteins, Bioactives, Markers, and Receptors for Stem Cell Biology,Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology, Antibodies to Actin Isoforms, General Stem Cell Biology, |
biological source | mouse |
antibody form | purified immunoglobulin |
clone | 1A4, monoclonal |
form | buffered aqueous solution |
mol wt | antigen mol wt ~42 kDa |
species reactivity | rabbit, guinea pig, mouse, chicken, snake, sheep, goat, human, frog, rat, canine, bovine |
application(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using human tonsil or appendix |
isotype | IgG2a |
conjugate | FITC conjugate |
shipped in | dry ice |
storage temp. | −20°C |
Gene Information | human ... ACTA2(59) mouse ... Acta2(11475) rat ... Acta2(81633) |
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