Hydroxyphenyl Fluorescein 产品活性：Hydroxyphenyl Fluorescein (HPF) 是一种稳定的 ROS 荧光探针染料，比 H2DCFDA (HY-D0940) 的特异性与稳定性强。Hydroxyphenyl Fluorescein 通过羟基自由基，与细胞内过氧亚硝基反应，可产生强烈的绿色荧光。Hydroxyphenyl Fluorescein 可应用于荧光显微镜、高通量成像仪、荧光酶标仪或流式细胞仪。其荧光波长：Ex/Em=490/515 nm。 产品来源： https://www.medchemexpress.cn/hydroxyphenyl-fluorescein.html 研究领域：Others | Immunology/Inflammation | NF-κB | Metabolic Enzyme/Protease 作用靶点：Fluorescent Dye | Reactive Oxygen Species In Vitro: Preparation of HPF working solution 1.1 Preparation of the stock solution Dissolve 1 mg of HPF to obtain 10 mM of HPF. Note: It is recommended to store the stock solution at -20 °C -80 °C away from light and avoid repetitive freeze-thaw cycles. 1.2 Preparation of HPF working solution Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-10 μM of HPF working solution. Note: Please adjust the concentration of HPF working solution according to the actual situation. Cell staining 2.1 Cell preparation.For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. 2.2 Add 1 mL of HPF working solution, and then incubate at room temperature for 30 minutes. 2.3 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant. 2.4 Wash twice with PBS, 5 minutes each time. 2.5 Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer. In Vivo: The Hydroxyphenyl Fluorescein (HPF) probe (15 μl, 25 μM) is given intratesticularly in anaesthetized mice 20 min before IR (ionizing radiation). The accumulation of OH by the fluorescence signal emitted by the oxidized form of HPF is assessed. 相关产品：Acetylcysteine | H2DCFDA | Deferoxamine mesylate | MitoSOX Red | Elesclomol | Quercetin | BODIPY 493/503 | D-Luciferin potassium | L-Ascorbic acid | Mito-TEMPO | Nile Red | FITC | Mitoquinone mesylate | Sulforaphane | JC-1 | Propidium Iodide | DiR | L-Glutathione reduced | FITC-Dextran (MW 4000) | Trolox | Dihydroethidium | CFDA-SE | 2-NBDG | Dimethyl fumarate | Zinc Protoporphyrin | Calcein-AM | FX-11 | DiI 品牌介绍: MCE (MedChemExpress) 拥有数百种全球独家化合物，我们致力于为全球科研客户提供最新最全的高品质小分子活性化合物；10,000 多种高选择性抑制剂、激动剂涉及各热门信号通路及疾病领域；设有专业的实验中心和严格的质控、验证体系；提供 LC/MS、NMR、HPLC、手性分析、元素分析等各项质检报告，确保产品的高纯度、高品质；产品的生物活性多经各国客户实验验证；Nature, Cell, Science 等多种顶级期刊及制药专利收录了MCE客户的科研成果；专业团队跟踪最新的制药及生命科学研究进展，为您提供全球最新的活性化合物；与世界各大制药公司及知名科研机构建立了长期的合作。