疏水作用层析(HIC)
Cytiva 疏水预装柱
| 品牌 | 厂商性质 | 产地 | 货期 |
|---|---|---|---|
| Cytiva | 生产商 | 美国 | 现货 |
AI问答
配套的仪器设备？
可以搭配的相关耗材试剂？
产品参数
| 货号 | 规格 | CAS号 | 操作 |
|---|---|---|---|
| 17371912 | YILIMART - HiTrap 5 x 5 mL Capto Butyl ImpRes | 8851.00 | 询底价 |
| 17519401 | YILIMART - HITRAP PHENYL FF (LS), 5X 5ML | 5281.00 | 询底价 |
| 17548410 | YILIMART - HiScreen Capto Phenyl ImpRes | 2319.00 | 询底价 |
| 28411007 | YILIMART - HiTrap HIC Selection Kit, 7x1ml | 3386.00 | 询底价 |
| 17519301 | YILIMART - HITRAP PHENYL FF (HS), 5 X 5ML | 5272.00 | 询底价 |
| 17371910 | YILIMART - HiScreen Capto Butyl ImpRes | 2595.00 | 询底价 |
| 28992473 | YILIMART - HiScreen Capto Butyl | 1850.00 | 询底价 |
| 28992472 | YILIMART - HiScreen Capto Phenyl (high sub) | 1850.00 | 询底价 |
| 28926984 | YILIMART - HiScreen Butyl FF | 1693.00 | 询底价 |
| 28978242 | YILIMART - HiScreen Butyl HP | 1914.00 | 询底价 |
| 28950516 | YILIMART - HiScreen Phenyl HP | 1923.00 | 询底价 |
| 17135101 | YILIMART - HITRAP PHENYL HP, 5 X 1ML | 2457.00 | 询底价 |
| 29018184 | YILIMART - HiPrep Phenyl HP16/10 | 9651.00 | 询底价 |
| 17135901 | YILIMART - HITRAP OCTYL FF, 5 X 1 ML | 2374.00 | 询底价 |
| 17135701 | YILIMART - HITRAP BUTYL FF, 5 X 1 ML | 2374.00 | 询底价 |
| 17135501 | YILIMART - HITRAP PHENYL FF (HS),5 X 1ML | 2365.00 | 询底价 |
| 28926988 | YILIMART - HiScreen Phenyl FF, high sub | 1693.00 | 询底价 |
| 28936547 | YILIMART - HiPrep Butyl FF 16/10 | 7958.00 | 询底价 |
| 28936545 | YILIMART - HiPrep Phenyl FF (high sub) 16/10 | 7958.00 | 询底价 |
| 28411005 | YILIMART - HiTrap Butyl HP, 5 x 5ml | 5456.00 | 询底价 |
| 28411001 | YILIMART - HiTrap Butyl HP, 5x1ml | 2466.00 | 询底价 |
| 17519701 | YILIMART - HITRAP BUTYL FF, 5 X 5 ML | 5272.00 | 询底价 |
| 17519601 | YILIMART - HITRAP OCTYL FF, 5 X 5 ML | 5281.00 | 询底价 |
| 17519501 | YILIMART - HITRAP PHENYL HP, 5 X 5 ML | 5447.00 | 询底价 |
| 17545108 | YILIMART - HiTrap 5x1 ml Capto Phenyl (high sub) | 3506.00 | 询底价 |
| 17545908 | YILIMART - HiTrap 5x1 ml Capto Butyl | 3506.00 | 询底价 |
| 17548412 | YILIMART - HiTrap 5 x 5 ml Capto Phenyl ImpRes | 7995.00 | 询底价 |
| 17548411 | YILIMART - HiTrap 5x1 ml Capto Phenyl ImpRes | 3690.00 | 询底价 |
| 17371911 | YILIMART - HiTrap 5x1 mL Capto Butyl ImpRes | 4122.00 | 询底价 |
| 29321087 | YILIMART - HiTrap Capto HIC Selection Kit （5x1ml） | 5622.00 | 询底价 |
| 28936548 | YILIMART - HiPrep Octyl FF 16/10 | 7958.00 | 询底价 |
| 28926989 | YILIMART - HiScreen Phenyl FF, low sub | 1684.00 | 询底价 |
| 28926986 | YILIMART - HiScreen Octyl FF | 1684.00 | 询底价 |
| 28926985 | YILIMART - HiScreen Butyl-S FF | 1684.00 | 询底价 |
| 17545909 | YILIMART - HiTrap 5x5 ml Capto Butyl | 7590.00 | 询底价 |
| 17545109 | YILIMART - HiTrap 5x5 ml Capto Phenyl (high sub) | 7590.00 | 询底价 |
| 17097813 | YILIMART - HITRAP BUTYL-S FF, 5 X 1ML | 2374.00 | 询底价 |
| 17135301 | YILIMART - HITRAP PHENYL FF (LS), 5X1ML | 2365.00 | 询底价 |
| 90100113 | YILIMART - HITRAP 5X5ML HIC KIT | 5576.00 | 询底价 |
| 17118601 | YILIMART - RESOURCE PHE 1 ML | 9246.00 | 询底价 |
| 17118501 | YILIMART - RESOURCE ISO 1 ML | 9246.00 | 询底价 |
产品介绍
疏水层析（HIC）的分离是基于蛋白质的疏水性氨基酸与基质的疏水性配基之间的可逆性相互作用。
蛋白质和多肽的疏水氨基酸大多埋藏在蛋白质分子结构的内部。然而，仍然有一些疏水性氨基酸会暴露在蛋白分子的表面与基质的疏水性配基进行相互作用。与反相层析相比，疏水作用层析基质上的配体浓度要低很多，洗脱条件也更加温和，从而有助于保持生物分子的生物活性。HIC特别适合于纯化经过硫酸铵沉淀或高盐洗脱后的样品，高离子强度可以加强蛋白与填料配基之间的疏水相互作用。
高离子强度的缓冲液可以增强疏水相互作用，这使得HIC成为硫酸铵沉淀和离子交换层析的一个卓越的纯化步骤。HIC非常适合于纯化工艺中的捕获或中纯步骤。
HIC期间，样品成分在高离子强度的缓冲液（典型的是1~2M的硫酸铵或者是3M的NaCl）中结合到填充柱上。高浓度的盐，尤其是硫酸铵，可能会沉淀蛋白质。因此，需要检测将要使用的结合条件下靶蛋白的溶解性。通常采用降低的盐梯度对靶蛋白进行连续梯度或分布梯度洗脱。
,Cytiva 疏水预装柱信息由Cytiva（思拓凡)为您提供，如您想了解更多关于Cytiva 疏水预装柱报价、型号、参数等信息，欢迎来电或留言咨询。
注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途
