X-GAL | MedChemExpress (MCE)

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X-GAL

CAS No. : 7240-90-6

MCE 国际站：X-GAL

产品活性：X-GAL (BCIG) 是一种广泛使用的显色 β-半乳糖苷酶底物。在被 β-半乳糖苷酶裂解之前，X-GAL 是一种无色的化合物，裂解后 X-GAL 变成一种不溶性且可检测的蓝色化合物，这使得 X-GAL 可用于细菌克隆的蓝白筛选等技术中。X-GAL也可用于检测 β-半乳糖苷酶的活性。

研究领域：Others  |  Metabolic Enzyme/Protease

作用靶点：Fluorescent Dye  |  Glucosidase

In Vitro: When the cells (HUVEC for example) were grown in dishes to 80% confluence, 50 μmol/L H₂O₂ were added in the medium for 12 h.
Subsequently, cells were harvested with trypsin/EDTA and washed three times with PBS and further fixed with 4% formaldehyde for 10 min.
As control, cells without H₂O₂ treatment were prepared in the same way. Cells were stained with by X-gal at 37 ℃.

The intestinal tissue was excised and cut longitudinally. After the intestinal content was rinsed off with phosphate buffer solution, the intestinal tissue was placed with the inner surface facing up on the slide.
Next, a single blue colony containing β-gal (E. coli DH5α containing pUC18) was dilution in 100 μL PBS and then 10 μL were added in the tissue.
After incubation for 1 h, the tissues were washed with PBS solution.
As control, tissues without the blue colony were prepared in the same way.
All tissues were fixed with 4% formaldehyde for 10 min and were further stained by adding X-gal at 37 ℃.

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