Toluidine Blue | 甲苯胺蓝O | MedChemExpress (MCE)

Toluidine Blue 产品活性：Toluidine Blue (Toluidine Blue O) 是一种碱性的醌亚胺染料 (活体染料)，对酸性组织成分有很高的亲和力，可将细胞核染成蓝色，多糖染成紫色。Toluidine Blue 对肥大细胞、粘蛋白和软骨细胞显示异染性。Toluidine Blue 可使植物组织和细胞的不同成分染成不同的颜色。Toluidine Blue 还可作为辅助诊断工具，鉴别恶性病变，如癌症。 产品来源： https://www.medchemexpress.cn/toluidine-blue.html 研究领域：Others 作用靶点：Fluorescent Dye In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Detection of infiltrating Mast cells: 1. Tissue processing 1) Immersed tissue samples in 10% buffered formalin for 24 h. 2) Remove tissues from the fixative and place in 70% alcohol. 3) Embed in paraffin: sequentially incubate in 70, 85, 95, and 100% ethanol, xylene, and molten paraffin (30 min incubation each), and set tissues in paraffin blocks. 4) Cut paraffin-embedded tissue in 5-7 μm sections and place onto glass slides. 5) Bake the slides for a minimum of 1 h at 55°C before use. 2. Tissue staining 1) De-wax paraffin-embedded tissue sections by immersing slides 5 min in xylene (or Histo-Clear), and rehydrate tissue by sequential immersions (5 min each) in 100%, 95%, and 70% ethanol and a final 2 min immersion in distilled water. 2) Place the slides in Harris’ hematoxylin solution for 70 s. 3) Briefly wash the excess of hematoxylin by immersing the slides two or three times in clean tap water. Briefly tap the slides on paper towels to remove excess water. 4) Immerse slides briefly three times in 70% alcohol, and wash again in clean tap water for 3 min. Remove excess water. 5) Place slides in Scott’s bluing solution for up to a minute. This step gently transforms hematoxylin into an insoluble blue color within the nucleus. 6) Inspect the slides under the bright-light microscope to ensure the nuclei are properly stained. 7) Wash slides in clean tap water for 3 min, and remove excess water. 8) Immerse slides in clean ~1.1% Toluidine Blue solution for 4 min.(Note 1). 9) Wash slides in clean tap water for 3 min and remove excess water. 10) Quickly dip the slides three times in one batch of 70% ethanol and then dip them five times in 5% eosin solution. 11) Dehydrate the slides by successive immersion in 70% (2 min), 95% (twice, 2 min each), and 100% ethanol (twice, 2 min each); conclude this step by immersing the slides in xylene (or Histo-Clear) (twice, 3 min each). 12) Permanently mount each slide with a clean coverslip using xylene-based mounting medium. 13) Let slides dry for a minimum of 60 min before analyzing them under the bright-light microscope. Note 1: 1.1% Toluidine Blue solution: mix 1.1 g Toluidine Blue with 100 mL 0.1 M sodium acetate buffer pH 4. Stir well. Adjust the pH to 2.0-2.5 by adding drops of 1 M hydrochloric acid. Staining is performed at 25 ℃, protected from light. Ready to use. Application of Toluidine Blue: 1. Connective tissue mucins, especially acid mucins. The tissue stains purple to red, while the background is stained blue. 2. Mast cell granules stain purple in color due to the presence of heparin and histamine. 3. Amyloid stain blue but under polarized light they give a bright red birefringence. 4. Endocrine cell granules are stained purple to red (concentration of stain is 0.01%). 5. Sulfatides stain red brown or yellow. Only lipids that are sufficiently acidic to induce a metachromatic shift are stained. 6. Corneybacterium diphtheria contains granules with polymerized inorganic polyphosphate, which stains red violet color. 7. Helicobacter stains dark blue against a variably blue background (concentration of stain is 1%). 8. Toluidine Blue can be used to stain frozen section because of the rapidity of the staining procedure (10-20 s) and better clarity of the cells. 9. Toluidine Blue can also dye the lignins of the plant blue-green, the phloem blue-purple, and the rest of the plant pale blue-green. 相关产品：MitoSOX Red | BODIPY 493/503 | D-Luciferin potassium | Nile Red | FITC | JC-1 | Propidium Iodide | DiR | FITC-Dextran (MW 4000) | Dihydroethidium | CFDA-SE | 2-NBDG | Calcein-AM | DiI | Thiazolyl Blue | Cy5.5 | TMRE | Fluo-4 AM | CY5 | Indocyanine green | PKH 26 | DiD perchlorate | Acridine Orange hydrochloride | BODIPY 581/591 C11 | FM4-64 | DiO | CY5-SE 品牌介绍: MCE (MedChemExpress) 拥有数百种全球独家化合物，我们致力于为全球科研客户提供最新最全的高品质小分子活性化合物；10,000 多种高选择性抑制剂、激动剂涉及各热门信号通路及疾病领域；设有专业的实验中心和严格的质控、验证体系；提供 LC/MS、NMR、HPLC、手性分析、元素分析等各项质检报告，确保产品的高纯度、高品质；产品的生物活性多经各国客户实验验证；Nature, Cell, Science 等多种顶级期刊及制药专利收录了MCE客户的科研成果；专业团队跟踪最新的制药及生命科学研究进展，为您提供全球最新的活性化合物；与世界各大制药公司及知名科研机构建立了长期的合作。

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