罗红霉素 T1151

Product Introduction
Bioactivity


英文名: Roxithromycin

描述: Roxithromycin (RU-28965) 是半合成的大环内酯类抗生素。

激酶实验: The half-maximal inhibitory concentrations (IC50s) of Fenofibrate, statins (atorvastatin, lovastatin, pravastatin, simvastatin and simvastatin acid, the active form of simvastatin) and glipizide for recombinant human CYP1A2, CYP2B6, CYP2C8, CYP2C9, CYP2C19, CYP2D6, and CYP3A4 are determined using fluorometric CYP450 inhibition assays. Briefly, the drugs are dissolved in methanol or acetonitrile. In 96 well assay plates, the drugs are diluted to a series of concentrations in a solution containing cofactors including NADP+ (final concentration 1.3 mM), MgCl2 (final concentration 3.3 m M), glucose-6-phosphate (G6P, final concentration 3.3 mM) and glucose 6-phosphate dehydrogenase (final concentration 0.4 U/mL). The mixture is pre-incubated at 37°C for 10 min. The enzymes and fluorogenic substrates are diluted to desired concentrations in sodium phosphate reaction buffer (pH 7.4, final concentration 200 mM) and mixed. Reactions are initiated with addition of the enzyme and substrate mixture to the cofactor and drug mixture. The final reaction volume of all assays is 200 μL. After incubating at 37°C for a pre-specified period of time (15 to 45 min), the reactions are stopped with addition of 75 μL quenching solution (0.5 M Tris base or 2N NaOH). Fluorescence is determined using a BioTek Synergy 2 fluorescence reader. Each of the drugs is tested at eight concentrations in duplicate. To estimate IC50s, percent of inhibition is calculated using net fluorescence that is corrected for the background. The values of percent of inhibition are then fitted to a three or four parameter log-logistic model[1].

存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度: Ethanol : 154 mg/mL (184 mM)
H2O : < 1 mg/mL (insoluble or slightly soluble)
DMSO : 60 mg/mL (71.68 mM)


关键字: Roxithromycin | RU 28965 | Inhibitor | Antibiotic | Bacterial | inhibit | RU28965

相关产品: Oxytetracycline | Kanamycin | Clarithromycin | Tigecycline | Puromycin dihydrochloride | Capreomycin sulfate | Hygromycin B | Methacycline hydrochloride | Chloramphenicol | Kanamycin sulfate

相关库: Drug Repurposing Compound Library | Approved Drug Library | Human Metabolite Library | Anti-Parasitic Compound Library | Natural Product Library for HTS | Microbial Natural Product Library | Orally Active Compound Library | Target-Focused Phenotypic Screening Library | NO PAINS Compound Library | Bioactive Compound Library

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注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途