化合物 T13540 T13540

Product Introduction
Bioactivity


英文名: alpha-Amanitin

描述: alpha-Amanitin is the principal toxin of poisonous mushrooms, exerting its toxic function by inhibiting RNA-polymerase II.

体外活性: Alpha-Amanitin decreases TAF15 mRNA and TAF15 protein levels in MKN45 cells and inhibits the RNAPII activity towards TAF15 mRNA [2]. alpha-Amanitin decreases cell viability by 14%, 21%, 41%, 44%, and 50% at concentrations of 100, 10, 1, 0.1, and 0.01 μg/mL, respectively. The LD50 of the alpha-Amanitin at 36 h is measured as 1 μg/mL. The total amount of protein within the cell at 24 h is significantly increased for the 1 μg/mL dose of alpha-Amanitin compared to the control [3]. Alpha-Amanitin dramatically decreases the expression of gap junctional genes and FSHr and LHR in cumulus cells [4].

体内活性: The intravenous LD50 dose of alpha-Amanitin is 0.327 mg/kg body weight after intravenous injection into BALB/c mice. After 12 h of alpha-Amanitin injection in the caudal vein, the levels of WBC, RBC, and HGB decrease significantly, while those of BUN and Crea increase significantly in serum. alpha-Amanitin inhibits some genes (Irx4, Hsp90b1, etc.), whose encoded proteins regulate the RNA polymerase II activity. alpha-Amanitin down-regulates some proteins (Nmi, Trpc5, etc.) taking part in the transcription progress [1]. alpha-Amanitin has potent activity in DTC suppression. Mice injected with alpha-Amanitin (0.4?mg/kg, i.p.)-treated cells maintain their body weight, while those receiving a peritoneal injection of MKN45 cells show a constant decrease in body weight [2].

存储条件: store at low temperaturePowder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度: H2O : 33.33 mg/mL (36.27 mM)


关键字: alphaAmanitin | alpha-Amanitin | alpha-Amatoxin | a-Amanitin | alpha Amanitin | a-Amatoxin

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注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途