化合物Ac-DEVD-AMC T18909

Product Introduction
Bioactivity


英文名: Ac-DEVD-AMC

描述: Ac-DEVD-AMC (AC-ASP-MET-GLN-ASP-7-AMINO-4-METHYLCOUMARIN) 是一种 Caspase-3底物。

细胞实验: Lung fragments (about 20 mg each) are collected from rats and mice. The samples are incubated at 37°C in 50 mL KH buffer or MEM (continuously gass with 95% O2: 5% CO2) for up to 6 h. At specific time points, samples are incubated in oxygenated KH buffer or MEM with 32 μM zVAD-fmk or 15 μL DMSO for 20 min (f/v=1.0 mL). Ac-DEVD-AMC (37 μM) is then added and the incubation continues for an additional 20 min. At the end of incubation, the tissue is disrupted by vigorous homogenization for 2 min, sonication for 3 min, and 10 passages through a 27-G needle. This disruption procedure quenches the Ac-DEVD-AMC cleavage reaction due to dilution. The supernatants are collected by centrifugation (~6,300g for 90 min) through a microcentrifuge filter, separated on HPLC, and analyzed for the fluorogenic AMC moiety [2].

体外活性: The AMC moiety is highest at 2 h with an area?of 5.6±1.8, the AMC peak area for Ac-DEVD-AMC incubated without a lung specimen (substrate alone) for 6 h is relatively small [1]. Ac-DEVD-AMC can be used to monitor intracellular caspase-3 activity[2].

存储条件: keep away from moisturePowder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度: DMSO : ≤ 10 mg/mL (14.80 mM)
H2O : < 0.1 mg/mL (insoluble)


关键字: Ac-DEVD-AMC | Ac DEVD AMC | AcDEVDAMC | Inhibitor | inhibit | Caspase

相关产品: Z-WEHD-FMK | Crustecdysone | TC11 | Marginatoxin | Taurodeoxycholic acid | Salvianolic acid F | Daphnoretin | Penicillic acid | SKLB-163 | PAC-1

相关库: Anti-Neurodegenerative Disease Compound Library | Pyroptosis Compound Library | Bioactive Compounds Library Max | Peptide Compound Library | Apoptosis Compound Library | Bioactive Compound Library

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注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途