化合物RG108 T2038

Product Introduction
Bioactivity


英文名: RG108

描述: RG108 (N-Phthalyl-L-tryptophan) 是一种非核苷的 DNA 甲基转移酶 (DNMTs) 抑制剂，IC50为115 nM。它阻断 DNA 甲基转移酶的活性位点，可引起抑癌基因的去甲基化和再激活。

细胞实验: For the determination of cellular growth and viability, cells are stained with trypan blue and counted using a standard counting grid.(Only for Reference)

激酶实验: In vitro methylation assay: The substrate DNA for the in vitro methylation assay is a 798 bp fragment (?423/+375 relative to the initiation codon) from the promoter region of the human p16Ink4a gene. The methylation reaction contains 350 to 400 ng substrate DNA and 4 units of M.SssI methylase (0.5 μM) in a final volume of 50 μL. Inhibitors are added to final concentrations of 10, 100, 200, and 500 μM, respectively. Reactions are done at 37 °C for 2 hours. After completion, the reaction is inactivated at 65 °C for 15 minutes and the DNA is purified using PCR Purification kit. Three hundred nanograms of purified DNA is digested for 3 hours at 60 °C with 30 units of BstUI and analyzed on 2% Tris-borate EDTA agarose gels.

体内活性: SM衍生的iPS细胞中,RG108能够明显降低DNA活性。在肿瘤细胞中,RG108抑制基因脱甲基和再活化,但是不影响着丝粒基因序列的甲基化。

存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度: Ethanol : 33.4 mg/mL (100 mM)
DMSO : 33.4 mg/mL (100 mM)


关键字: inhibit | DNA Methyltransferase | Inhibitor | DNMTs | DNA MTases | RG 108 | RG108 | RG-108

相关产品: CM-579 | Decitabine | 6-Thioguanine | Bobcat339 | 2',3',5'-triacetyl-5-Azacytidine | Neurodazine | TFMB-(S)-2-HG | SW155246 | Zebularine | Tetrahydrouridine

相关库: Anti-Aging Compound Library | Bioactive Compounds Library Max | Inhibitor Library | Epigenetics Compound Library | Target-Focused Phenotypic Screening Library | Methylation Compound Library | Stem Cell Differentiation Compound Library | NO PAINS Compound Library | Bioactive Compound Library | Chromatin Modification Compound Library

化合物RG108 T2038信息由TargetMol中国为您提供，如您想了解更多关于化合物RG108 T2038报价、型号、参数等信息，欢迎来电或留言咨询。

注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途