化合物GW 4064 T2233

Product Introduction
Bioactivity


英文名: GW 4064

描述: GW 4064 是一种有效的法尼醇 X 受体 (FXR) 激动剂，EC50为 65 nM。

细胞实验: GW 4064 is dissolved in DMSO and stored, and then diluted with appropriate media before use[2]. Mouse liver cells (BNL CL.2) are maintained in a humidified incubator under 5% CO2 at 37°C in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% Penicillin/Streptomycin. When cells are divided into six-well plates and reach ~90% confluence, sub-confluent cells are washed three times with phosphate buffered saline (PBS) and replaced with serum-free DMEM supplemented with 1% fatty acid-free BSA. Oleic acid (final concentration 500 μM) and GW4064 at various concentrations are added and incubated for 24 h. Cells are then fixed with 4% formaldehyde for Oil Red O staining or harvested for protein and western blot analysis[2].

体外活性: 在小鼠FXR表达载体转染的CV-1细胞中（EC50=80 nM）,在人FXR表达载体转染的CV-1细胞中（EC50=90 nM）,GW 4064作为选择性非甾体FXR激动剂,能够发挥激动作用。

体内活性: 在Fisher大鼠中,口服给药GW 4064（ED50 = 20 mg/kg）,能够降低大鼠血清中的甘油三酯.

存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度: DMSO : 50 mg/mL (92.11 mM)
Ethanol : 5.4 mg/mL (10 mM)


关键字: Inhibitor | FXR | Autophagy | GW 4064 | inhibit | NR1H4 | GW-4064 | GW4064

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相关库: Bioactive Lipid Compound Library | ReFRAME Related Library | Anti-Metabolism Disease Compound Library | Bioactive Compounds Library Max | Nuclear Receptor Compound Library | Lipid Metabolism Compound Library | Autophagy Compound Library | Metabolism Compound Library | NO PAINS Compound Library | Bioactive Compound Library

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注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途